|Recombinant Ferret TNFA / TNF-alpha protein (Catalog#60002-FNAE)|
|0.2 μm filtered solution in PBS with 5% trehalose|
|This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Ferret TNFA / TNF-alpha (rF TNFA / TNF-alpha; Catalog#60002-FNAE; A3FBF1; Val 77-Leu 233). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.|
Has cross-reactivity in ELISA with
No cross-reactivity in ELISA with
E.coli cell lysate
ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Ferret TNFα. The detection limit for Ferret TNFα is approximately 0.078 ng/well.
ELISA(Det): 0.1-1 µg/ml
This antibody will detect Ferret TNF-alpha / TNFA / TNFSF2 in ELISA pair set (Catalog: # SEK60002). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 60002-R303).
Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.