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TNF-alpha / TNFA / TNFSF2 / TNFSF2 Antibody, Rabbit PAb, Antigen Affinity Purified

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Human TNF Antibody Product Information
Immunogen:Recombinant Human TNF-alpha / TNFa / TNFSF2 protein (Catalog#10602-HNAE)
Clone ID:
Ig Type:Rabbit IgG
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:Produced in rabbits immunized with purified, recombinant Human TNF-alpha / TNFa / TNFSF2 (rh TNF-alpha / TNFa / TNFSF2; Catalog#10602-HNAE; NP_000585.2; Val77-Leu233). TNF-alpha / TNFa / TNFSF2 specific IgG was purified by Human TNF-alpha / TNFa / TNFSF2 affinity chromatography.
Human TNF Antibody Usage Guide
Specificity:Human TNF-alpha / TNFa / TNFSF2
Application:WB, ELISA, IP

WB: 5-10 μg/mL

ELISA: 0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human TNF-alpha / TNFa / TNFSF2. The detection limit for Human TNF-alpha / TNFa / TNFSF2 is approximately < 0.039 ng/well.

IP: 1-4 μg/mg of lysate

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human TNF Antibody WB Application Image
TNF-alpha / TNFA / TNFSF2 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
Human TNF Antibody IP Application Image
TNF-alpha / TNFA / TNFSF2 Antibody, Rabbit PAb, Antigen Affinity Purified
Other TNF Antibody Products
TNF-alpha/TNFA Background

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

TNF-alpha Protein & Antibody

Human TNF-alpha/TNFA References
  • Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
  • Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"