Anti-TNF-alpha Antibody

Cat: 10602-RP02

Contact us:

215-583-7898
order@sinobiological.com
Distributors

All TNF-alpha Reagents

CRO Services

Product recommended


All TNF-alpha Reagents

Anti-TNF-alpha Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-TNF-alpha Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human TNF-alpha
Immunogen
Recombinant Human TNF-alpha / TNFa / TNFSF2 protein (Catalog#10602-HNAE)
Preparation
Produced in rabbits immunized with purified, recombinant Human TNF-alpha / TNFa / TNFSF2 (rh TNF-alpha / TNFa / TNFSF2; Catalog#10602-HNAE; NP_000585.2; Val77-Leu233). TNF-alpha / TNFa / TNFSF2 specific IgG was purified by Human TNF-alpha / TNFa / TNFSF2 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Anti-TNF-alpha Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-TNF-alpha Antibody (Rabbit Polyclonal antibody) Images

Human TNF-alpha/TNFA/TNFSF2 Immunoprecipitation(IP) 8003

TNFa was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

Lane B:0.5 mg HepG2 Whole Cell Lysate

Lane C:0.5 mg SW480 Whole Cell Lysate

2 µL anti-TNFa rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-TNFa rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 23 kDa

Observed band size: 33 kDa

Human TNF-alpha/TNFA/TNFSF2 Western blot (WB) 13252

Anti-TNF-alpha rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:25 kDa

Observed band size:27 kDa

Anti-TNF-alpha Antibody Alternative Names

Anti-DIF Antibody;Anti-TNF-alpha Antibody;Anti-TNFA Antibody;Anti-TNFSF2 Antibody

TNF-alpha Background Information

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

TNF-alpha Protein & Antibody

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
tumor necrosis factor
Research Areas
References
  • Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
  • Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-TNF-alpha Antibody Rabbit Monoclonal ELISA 70003-R001
    Anti-TNF-alpha Antibody Mouse Monoclonal ELISA,ELISA(Det) 60002-MM11
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 70003-RP01
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 70003-RP02
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 60002-T16
    Anti-TNF-alpha Antibody Rabbit Monoclonal ELISA(Cap) 60002-R303
    Anti-TNF-alpha Antibody Rabbit Monoclonal ELISA 60002-R030
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 90330-RP01
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 60002-RP01
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 80045-RP01
    Anti-TNF-alpha Antibody Rabbit Polyclonal WB,ELISA,ICC/IF,IF 80045-T50
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 80045-T16
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 80045-RP02
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 50349-RP01
    Anti-TNF-alpha Antibody Rabbit Monoclonal ELISA,ELISA(Cap) 50349-R023
    Anti-TNF-alpha Antibody Rabbit Monoclonal ELISA 50349-R009
    TNF-alpha/TNFA/TNFSF2 Neutralizing Antibody Rabbit Monoclonal Neutralization 50349-RN023
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 50349-T16
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 50349-RP02
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 10602-RP01
    Anti-TNF-alpha Antibody Rabbit Monoclonal ELISA 10602-R211
    Anti-TNF-alpha Antibody Mouse Monoclonal ELISA,ELISA(Det) 10602-MM08
    Anti-TNF-alpha Antibody Rabbit Polyclonal WB,ELISA 10602-RP03
    Anti-TNF-alpha Antibody (APC) Rabbit Monoclonal FCM 10602-R101-A
    Anti-TNF-alpha Antibody Rabbit Monoclonal FCM 10602-R101
    Anti-TNF-alpha Antibody (FITC) Mouse Monoclonal FCM 10602-MM01-F
    Anti-TNF-alpha Antibody (PE) Rabbit Monoclonal FCM 10602-R101-P
    Anti-TNF-alpha Antibody Mouse Monoclonal ELISA,ELISA(Cap),FCM 10602-MM01
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 10602-T16
    Anti-TNF-alpha Antibody Rabbit Monoclonal ELISA 10602-RE23
    Anti-TNF-alpha Antibody (FITC) Rabbit Monoclonal FCM 10602-R101-F
    TNF-alpha / TNFA Neutralizing Antibody Mouse Monoclonal Neutralization 10602-MM0N1
    TNF-alpha / TNFA / TNFSF2 Neutralizing Antibody Rabbit Monoclonal FCM,Neutralization 10602-R10N1
    info info
    添加购物车成功! 添加购物车失败!