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TNF-alpha / TNFA / TNFSF2 Neutralizing Antibody

  • TNF-alpha / TNFA Neutralizing Antibody
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Human TNF Antibody Product Information
Immunogen:Recombinant Human TNF-alpha / TNFA protein (Catalog#10602-HNAE)
Clone ID:R101
Ig Type:Rabbit IgG
Endotoxin:Please contact us for more information.
Formulation:0.2 μm filtered solution in PBS
Preparation:This antibody was obtained from a rabbit immunized with purified, recombinant Human TNF-alpha / TNFA (rHuman TNF-alpha / TNFA; Catalog#10602-HNAE; NP_000585.2; Val77-Leu233) and was produced using recombinant antibody technology.
Human TNF Antibody Usage Guide
Specificity:Human TNF-alpha / TNFA
Has cross-reactivity with Cynomolgus TNFa (Catalog # 90330-CNAE) /Canine TNFa Catalog # (70003-DNAE) and Ferret TNFa (Catalog # 60002-FNAE)
No cross-reactivity with Mouse TNFa (Catalog # 50349-MNAE ) and Rat TNFa (Catalog # 80045-RNAE) in ELISA assay

In a functional ELISA which immobilized recombinant Human TNFR1 (Catalog # 10872-H08H) at 1μg/mL (100 µL/well) in the plate, the Rabbit Anti-Human TNFα Monoclonal Antibody (Catalog # 10602-R10N1) can block the binding of 0.2 μg/mL of biotinylated Human TNFα (Catalog # 10602-HNAE) ) to human TNFR1,the EC50 is 1.25 μg/mL;In a functional ELISA which immobilized recombinant Human TNFR2 (Catalog # 10417-H08H) at 2 μg/mL (100 µL/well) in the plate, the Rabbit Anti-Human TNFα Monoclonal Antibody (Catalog # 10602-R10N1) can block the binding of 0.2 μg/mL of biotinylated Human TNFα (Catalog # 10602-HNAE) ) to human TNFR1,the EC50 is 1.26 μg/mL.

The neutralization activity of TNFa Neutralizing Antibody is Measured by its ability to neutralize TNF-α induced cytotoxicity in the L-929 mouse fibroblast cell line. The Neutralization titer (IC50) is typically 8.8-35.2 ng/mL in the presence of 2 ng/mL Recombinant Human TNF-α (10602-HNAE) and 1 μg/mL actinomycin D.

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human TNF Antibody Neutralization Application Image
TNF-alpha / TNFA Neutralizing Antibody
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Cytotoxicity Induced by TNFα was Neutralized by Human TNFα Antibody. Recombinant Human TNFα (Catalog 10602-HNAE) induces cytotoxicity in the the L929 mouse fibroblast cell line. Cytotoxicity elicited by 2 ng/mL of Recombinant Human TNFα is neutralized by increasing concentrations of Anti Human TNFα neutralizing Rabbit Antibody (Catalog 10602-R101). The IC50 is typically 8.8-35.2 ng/mL in the presence of the metabolic inhibitor actinomycin D (1 μg/mL).
Other TNF Antibody Products
Immunochemical staining of human CD40 in human tonsil with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
TNF-alpha/TNFA/TNFSF2 Background

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

TNF-alpha Protein & Antibody

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Human TNF-alpha/TNFA/TNFSF2 References
  • Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
  • Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
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    Catalog: 10602-R10N1-200
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    Datasheet & Documentation

    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.