TNF-alpha Protein & Antibody (TNFA/TNFSF2)

Tumor Necrosis Factor alpha/TNF Superfamily, member 2

Sino Biological offers a comprehensive set of tools for TNF alpha related research. We offer active TNF alpha proteins from human, mouse, and canine, TNF alpha antibodies, TNF alpha ELISA kit, and TNF alpha ORF cDNA clones.

TNF-alpha Background

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

TNF-alpha Protein & Antibody

TNF-alpha Protein, Recombinant

Molecule	Species	Description	Cat. No
TNF-alpha	Human	TNF-alpha Protein, Native	10602-HNAE
TNF-alpha	Mouse	TNF-alpha Protein, Native	50349-MNAE
TNF-alpha	Rat	TNF-alpha Protein, Native	80045-RNAE
TNF-alpha	Cynomolgus	TNF-alpha Protein, Native	90018-CNAE
TNF-alpha	Canine	TNF-alpha Protein, Native	70003-DNAE
TNF-alpha	Ferret	TNF-alpha Protein, Native	60002-FNAE

  10602-HNAE:  The ED₅₀ for this effect is typically 25-200 pg/ml, corresponding to a specific activity of 5x10⁶-4x10⁷ units / mg. Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.

  50349-MNAE:  The ED₅₀ for this effect is typically 0.04-0.08 ng/mL. Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.

  80045-RNAE: The ED₅₀ for this effect is typically 10-20 pg/mL. Measured in a cytotoxicity assay using L930 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.

  90018-CNAE:  The ED₅₀ for this effect is typically 68.4 pg/ml. Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.

  70003-DNAE:  The ED₅₀ for this effect is typically 0.2-1.2 ng/ml. Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.

  60002-FNAE: The EC₅₀ for this effect is typically 50-200 pg/ml. Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.

TNF-alpha Antibody

Molecule	Application	Description	Cat. No
Human TNF-alpha	ELISA	Mouse Monoclonal Antibody	10602-MM01
Human TNF-alpha	WB, ELISA	Rabbit Monoclonal Antibody	10602-R211
Human TNF-alpha	ELISA	Rabbit Polyclonal Antibody	10602-RP03
Mouse TNF-alpha	WB, ELISA	Rabbit Polyclonal Antibody	50349-RP01
Mouse TNF-alpha	WB, ELISA	Rabbit Polyclonal Antibody (Antigen Affinity Purified)	50349-RP02

TNF-alpha Related Areas

Cancer>>Angiogenesis>>Cytokines/Chemokines in Angiogenesis>>TNF-alpha

Cancer>>Cancer Biomarkers>>TNF-alpha

Immunology>>Cytokine & Receptor>>TNF Superfamily>>TNF-alpha

TNF-alpha Alternative Names

TNF-alpha, TNF, TNFA, TNFSF2, DADB-70P7.1, DIF [Homo sapiens]

TNF-alpha, Tnf, Tnfa, TNFSF2, TNFalpha, Tnfsf1a, DIF, MGC151434 [Mus musculus]

TNF-alpha Related Products

Protein Products

Molecule	Species	Description
TNFSF7/CD70/CD27L	Human	TNFSF7/CD70/CD27L/Fc Protein, Recombinant
TNFSF9/4-1BBL/CD137L	Mouse	TNFSF9/4-1BBL/CD137L Protein, Recombinant
TNFSF14/LIGHT/CD258	Human	TNFSF14/LIGHT/CD258 Protein, Recombinant

Antibody Products

Molecule	Application	Description
Mouse TNFSF9/4-1BBL/CD137L	ELISA	Rabbit Polyclonal Antibody

cDNA Clone Products

Molecule	Species	Description
EDA-A1/Ectodysplasin-A1	Human	Homo sapiens EDA-A1 transcript variant 1 cDNA Clone(NM_001399.4)
TNF-beta/TNFSF1/Lymphotoxin alpha	Human	Homo sapiens TNF-beta/TNFSF1/Lymphotoxin alpha cDNA Clone(NM_000595.2)
TNF-alpha	Human	Homo sapiens TNF-alpha cDNA Clone(NM_000594.2)
TNF-alpha	Canine	Canis lupus familiars TNF-alpha cDNA Clone
TNFSF3/TNFC/lymphotoxin beta	Human	Homo sapiens TNFSF3/TNFC/lymphotoxin beta cDNA Clone(NM_002341.1)
TNFSF5/CD40L/CD154	Human	Homo sapiens TNFSF5/CD40L/CD154 cDNA Clone(NM_000074.2)
TNFSF6/CD95L/CD178	Human	Homo sapiens TNFSF6/CD95L/CD178 cDNA Clone(NM_000639.1)
TNFSF7/CD70/CD27L	Human	Homo sapiens TNFSF7/CD70/CD27L cDNA Clone(NM_001252.3)
TNFSF8/CD153/CD30L	Human	Homo sapiens TNFSF8/CD153/CD30L cDNA Clone(NM_001244.2)
TNFSF10/CD253/TRAIL	Human	Homo sapiens TNFSF10/CD253/TRAIL cDNA Clone(NM_003810.2)
TNFSF13/CD256	Human	Homo sapiens TNFSF13/CD256 cDNA Clone(NM_003808.3)
TNFSF13B/CD257	Human	Homo sapiens TNFSF13B/CD257 cDNA Clone(NM_006573.3)
TNFSF14/LIGHT/CD258	Human	Homo sapiens TNFSF14/LIGHT/CD258 transcript variant 1 cDNA Clone(NM_003807.2)

TNF-alpha Related Studies

1. Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
2. Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.