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Human TNF-alpha / TNFA / TNFSF2 ELISA Pair Set

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Human TNFa Materials provided
Capture Ab:0.5 mg/mL of mouse anti-Human TNF-alpha / TNFA / TNFSF2 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating.
Detection Ab:0.2 mg/mL of mouse anti-Human TNF-alpha / TNFA / TNFSF2 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 1.5 μg/mL in detection antibody dilution buffer before use.
Standard:Each vial contains 45 ng of recombinant Human TNF-alpha / TNFA / TNFSF2. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 600 pg/mL is recommended.
Human TNFa Sensitivity
The minimum detectable dose of Human TNF-alpha / TNFA / TNFSF2 was determined to be approximately 9.38 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Human TNFa Principle of the product
The Human TNF-alpha / TNFA / TNFSF2 ELISA Pair Set is for the quantitative determination of Human TNF-alpha / TNFA / TNFSF2.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human TNF-alpha / TNFA / TNFSF2 coated on a 96-well plate. Standards and samples are added to the wells, and any Human TNF-alpha / TNFA / TNFSF2 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-Human TNF-alpha / TNFA / TNFSF2 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human TNF-alpha / TNFA / TNFSF2 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
Human TNFa Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.

Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!

Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Human TNFa ELISA Pair Set Standard Curve
TNF-alpha/TNFA/TNFSF2 Background

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

TNF-alpha Protein & Antibody

Human TNF-alpha/TNFA/TNFSF2 References
  • Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
  • Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
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