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TNF-alpha / TNFA Antibody, Rabbit PAb, Antigen Affinity Purified

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Canine TNFa Antibody Product Information
Immunogen:Recombinant Canine TNF-alpha protein (Catalog#70003-DNAE)
Clone ID:
Ig Type:Rabbit IgG
Concentration:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:Produced in rabbits immunized with purified, recombinant Canine TNF-alpha (rCanine TNF-alpha; Catalog#70003-DNAE; NP_001003244.4; Val 77-Leu 233). TNF-alpha specific IgG was purified by Canine TNF-alpha affinity chromatography.
Canine TNFa Antibody Usage Guide
Specificity:Canine TNF-alpha
Application:ELISA

ELISA: 0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Canine TNFα. The detection limit for Canine TNFα-His is approximately 0.00245 ng/well.

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
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    Reactivity: Human  
    Application: ELISA  
      10602-R211-50
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      50 µg 
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      Reactivity: Human  
      Application: FCM  
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      10602-MM01-F-50
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      50 Tests 
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      Reactivity: Human  
      Application: FCM  
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      10602-R101-F-50
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      Reactivity: Human  
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        10602-T16-100
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        100 µg 
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        Reactivity: Human  
        Application: ELISA  
          10602-RE23-50
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          50 µg 
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          Reactivity: Mouse  
          Application: ELISA  
            50349-RP01-400
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            Reactivity: Mouse  
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              50349-RP02-200
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              Reactivity: Mouse  
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                50349-R009-50
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                50 µg 
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                Reactivity: Rat  
                Application: ELISA  
                  80045-RP01-200
                  80045-RP01-100
                  80045-RP01-400
                  200 µg 
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                  Application: ELISA  
                    80045-RP02-50
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                    Reactivity: Cynomolgus  
                    Application: ELISA  
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                      Reactivity: Canine  
                      Application: ELISA  
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                        50 µg 
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                        Reactivity: Canine  
                        Application: ELISA  
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                          100 µg 
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                          Reactivity: Canine  
                          Application: ELISA  
                            70003-MM04-200
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                            200 µg 
                            50 µg 
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                            Reactivity: Ferret  
                            Application: ELISA  
                              60002-T16-100
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                              100 µg 
                              50 µg 
                              200 µg 
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                              Reactivity: Ferret  
                              Application: ELISA  
                                60002-R030-50
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                                50 µg 
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                                Reactivity: Ferret  
                                Application: ELISA  
                                  60002-MM11-200
                                  60002-MM11-50
                                  60002-MM11-100
                                  200 µg 
                                  50 µg 
                                  100 µg 
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                                  Reactivity: Ferret  
                                  Application: ELISA  
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                                    60002-RP01-400
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                                    TNF-alpha/TNFA/TNFSF2 Background

                                    Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

                                    TNF-alpha Protein & Antibody

                                    Canine TNF-alpha/TNFA/TNFSF2 References
                                  • Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
                                  • Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
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                                    Catalog: 70003-RP02-200
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                                    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
                                    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"