Anti-TNF-alpha Antibody

Cat: 70003-R001
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All TNF-alpha Reagents
Anti-TNF-alpha Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-TNF-alpha Antibody
Validated applications
ELISA
Species reactivity
Reacts with: Canine
Specificity
Canine TNF-alpha
Has cross-reactivity in ELISA with Human TNF-a / TNF-alpha
No cross-reactivity in ELISA with Mouse TNF-a / TNF-alpha
Immunogen
Recombinant Canine TNF-alpha Protein (Catalog#70003-DNAE)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Canine TNF-alpha (rCanine TNF-alpha; Catalog#70003-DNAE; NP_001003244.4; Val 77-Leu 233) .
Source
Monoclonal Rabbit IgG Clone #001
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-TNF-alpha Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:5000-1:10000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

TNF-alpha Background Information

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

TNF-alpha Protein & Antibody

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
tumor necrosis factor
Research Areas
References
  • Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
  • Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-TNF-alpha Antibody Rabbit Monoclonal ELISA 60002-R030
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    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 70003-RP01
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 70003-RP02
    Anti-TNF-alpha Antibody Mouse Monoclonal ELISA,ELISA(Det) 60002-MM11
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    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 80045-T16
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    Anti-TNF-alpha Antibody Rabbit Polyclonal WB,ELISA,ICC/IF,IF 80045-T50
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    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 50349-RP01
    Anti-TNF-alpha Antibody Rabbit Monoclonal ELISA 50349-R009
    Anti-TNF-alpha Antibody Rabbit Monoclonal ELISA(Cap) 50349-R023
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 50349-T16
    TNF-alpha/TNFA/TNFSF2 Neutralizing Antibody Rabbit Monoclonal Neutralization 50349-RN023
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 50349-RP02
    Anti-TNF-alpha Antibody Rabbit Polyclonal WB,ELISA,IP 10602-RP02
    TNF-alpha / TNFA / TNFSF2 Neutralizing Antibody Rabbit Monoclonal FCM,Neutralization 10602-R10N1
    Anti-TNF-alpha Antibody (APC) Rabbit Monoclonal FCM 10602-R101-A
    Anti-TNF-alpha Antibody Rabbit Polyclonal WB,ELISA 10602-RP03
    TNF-alpha / TNFA Neutralizing Antibody Mouse Monoclonal Neutralization 10602-MM0N1
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 10602-RP01
    Anti-TNF-alpha Antibody Rabbit Monoclonal ELISA 10602-R211
    Anti-TNF-alpha Antibody Mouse Monoclonal ELISA,ELISA(Det) 10602-MM08
    Anti-TNF-alpha Antibody (FITC) Mouse Monoclonal FCM 10602-MM01-F
    Anti-TNF-alpha Antibody Mouse Monoclonal ELISA,ELISA(Cap),FCM 10602-MM01
    Anti-TNF-alpha Antibody Rabbit Monoclonal ELISA 10602-RE23
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 10602-T16
    Anti-TNF-alpha Antibody (PE) Rabbit Monoclonal FCM 10602-R101-P
    Anti-TNF-alpha Antibody (FITC) Rabbit Monoclonal FCM 10602-R101-F
    Anti-TNF-alpha Antibody Rabbit Monoclonal FCM 10602-R101
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