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TNF-alpha / TNFA / TNFSF2 Antibody, Mouse MAb

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Human TNFa Antibody Product Information
Immunogen:Recombinant Human TNFα protein (Catalog#10602-HNAE)
Clone ID:9A7A5G9
Ig Type:Mouse IgG1
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Tumor necrosis factor-alpha (rhTNFα; Catalog#10602-HNAE; Val 77-Leu 233; NP_000585.2). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human TNFa Antibody Usage Guide
Specificity:Human TNFα
No cross-reactivity in WB and ELISA with
E.coli cell lysate
Application:ELISA, FCM

ELISA: 0.5-1.0 μg/mL

This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Human TNFα. The detection limit for Human TNFα is approximately 0.6 ng/well.

FCM: 0.5-2 μg/Test

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human TNFa Antibody FC Application Image
[Click to enlarge image]
Caption:
Flow cytometric analysis of Purified anti-Human TNFa on CHO transfected cells and stimulated lymphocytes. Left panel The transfected cells stained with Purified Mouse anti-TNFa (Bold line hisgram,1 μg/test), To demonstrate specificity of staining, the binding of 10602-MM01 was blocked by the preincubation of the purified antibody with molar excess of recombinant human TNFa (5 μg, Cat. No. 10602-HNAE) for 1 hour (dashed line hisgram), then stained with a FITC-conjugated second step antibody. Right panel Human PBMC were stimulated for 4 hrs with Leukocyte Activation Cocktail (with BD GolgiPlug™, BD Pharmingen™ Cat. No. 550583), then cells were harvested and treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714).
TNF-alpha / TNFA Antibody, Mouse MAb, Flow cytometric
[Click to enlarge image]
Caption:
Flow cytometric analysis of Purified anti-Human TNFa on CHO transfected cells and stimulated lymphocytes. Left panel The transfected cells stained with Purified Mouse anti-TNFa (Bold line hisgram,1 μg/test), To demonstrate specificity of staining, the binding of 10602-MM01 was blocked by the preincubation of the purified antibody with molar excess of recombinant human TNFa (5 μg, Cat. No. 10602-HNAE) for 1 hour (dashed line hisgram), then stained with a FITC-conjugated second step antibody. Right panel Human PBMC were stimulated for 4 hrs with Leukocyte Activation Cocktail (with BD GolgiPlug™, BD Pharmingen™ Cat. No. 550583), then cells were harvested and treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714).
TNF-alpha / TNFA Antibody, Mouse MAb, Flow cytometric
Other TNFa Antibody Products
TNF-alpha/TNFA Background

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

TNF-alpha Protein & Antibody

Human TNF-alpha/TNFA References
  • Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
  • Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
  • Product nameProduct name
    Size / Price
    Catalog: 10602-MM01-50
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    AvailabilityIn Stock
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    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"