Anti-TNF-alpha Antibody

Cat: 10602-MM01
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All TNF-alpha Reagents
Anti-TNF-alpha Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-TNF-alpha Antibody
Validated applications
ELISA,ELISA(Cap),FCM
Species reactivity
Reacts with: Human
Specificity
Human TNF-alpha
Immunogen
Recombinant Human TNFα protein (Catalog#10602-HNAE)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Tumor necrosis factor-alpha (rhTNFα; Catalog#10602-HNAE; Val 77-Leu 233; NP_000585.2). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #9A7A5G9
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-TNF-alpha Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  
FCM 1:25-1:100  
ELISA(Cap) 1:250-1:2000 This antibody will detect Human TNF-alpha / TNFA / TNFSF2 in ELISA pair set (Catalog: # SEKA10602). In a sandwich ELISA, it can be used as capture antibody when paired with (Catalog: # 10602-MM08).

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-TNF-alpha Antibody (Mouse Monoclonal antibody) Images
Human TNF-alpha/TNFA/TNFSF2 Flow Cytometry (FC) 4907
Flow cytometric analysis of Purified anti-Human TNFa on CHO transfected cells and stimulated lymphocytes. Left panel The transfected cells stained with Purified Mouse anti-TNFa (Bold line hisgram), To demonstrate specificity of staining, the binding of 10602-MM01 was blocked by the preincubation of the purified antibody with molar excess of recombinant human TNFa (5 μg, Cat. No. 10602-HNAE) for 1 hour (dashed line hisgram), then stained with a FITC-conjugated second step antibody. Right panel Human PBMC were stimulated for 4 hrs with Leukocyte Activation Cocktail (with BD GolgiPlug™, BD Pharmingen™ Cat. No. 550583), then cells were harvested and treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714).
Anti-TNF-alpha Antibody Alternative Names
Anti-DIF Antibody;Anti-TNF-alpha Antibody;Anti-TNFA Antibody;Anti-TNFSF2 Antibody
TNF-alpha Background Information

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

TNF-alpha Protein & Antibody

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
tumor necrosis factor
Research Areas
References
  • Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
  • Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-TNF-alpha Antibody Rabbit Monoclonal ELISA(Cap) 60002-R303
    Anti-TNF-alpha Antibody Mouse Monoclonal ELISA,ELISA(Det) 60002-MM11
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 70003-RP01
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 60002-RP01
    Anti-TNF-alpha Antibody Rabbit Monoclonal ELISA 60002-R030
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 70003-RP02
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 90330-RP01
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 60002-T16
    Anti-TNF-alpha Antibody Rabbit Monoclonal ELISA 70003-R001
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 80045-RP02
    Anti-TNF-alpha Antibody Rabbit Polyclonal WB,ELISA,ICC/IF,IF 80045-T50
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 80045-T16
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 80045-RP01
    Anti-TNF-alpha Antibody Rabbit Monoclonal ELISA 50349-R009
    Anti-TNF-alpha Antibody Rabbit Monoclonal ELISA(Cap) 50349-R023
    TNF-alpha/TNFA/TNFSF2 Neutralizing Antibody Rabbit Monoclonal Neutralization 50349-RN023
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 50349-RP02
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 50349-T16
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 50349-RP01
    Anti-TNF-alpha Antibody (FITC) Rabbit Monoclonal FCM 10602-R101-F
    Anti-TNF-alpha Antibody Rabbit Monoclonal ELISA 10602-RE23
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 10602-T16
    Anti-TNF-alpha Antibody Rabbit Polyclonal WB,ELISA 10602-RP03
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 10602-RP01
    Anti-TNF-alpha Antibody (FITC) Mouse Monoclonal FCM 10602-MM01-F
    Anti-TNF-alpha Antibody (PE) Rabbit Monoclonal FCM 10602-R101-P
    Anti-TNF-alpha Antibody (APC) Rabbit Monoclonal FCM 10602-R101-A
    TNF-alpha / TNFA Neutralizing Antibody Mouse Monoclonal Neutralization 10602-MM0N1
    Anti-TNF-alpha Antibody Rabbit Polyclonal WB,ELISA,IP 10602-RP02
    TNF-alpha / TNFA / TNFSF2 Neutralizing Antibody Rabbit Monoclonal FCM,Neutralization 10602-R10N1
    Anti-TNF-alpha Antibody Rabbit Monoclonal ELISA 10602-R211
    Anti-TNF-alpha Antibody Mouse Monoclonal ELISA,ELISA(Det) 10602-MM08
    Anti-TNF-alpha Antibody Rabbit Monoclonal FCM 10602-R101
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