|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Recombinant Human TNFα protein (Catalog#10602-HNAE)|
|0.2 μm filtered solution in PBS with 5% trehalose|
|This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Tumor necrosis factor-alpha (rhTNFα; Catalog#10602-HNAE; Val 77-Leu 233; NP_000585.2). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.|
No cross-reactivity in WB and ELISA with
E.coli cell lysate
ELISA: 0.5-1.0 μg/mL
This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Human TNFα. The detection limit for Human TNFα is approximately 0.6 ng/well.
|This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.|
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Flow cytometric analysis of Purified anti-Human TNFa on CHO transfected cells and stimulated lymphocytes. Left panel The transfected cells stained with Purified Mouse anti-TNFa (Bold line hisgram,1 μg/test), To demonstrate specificity of staining, the binding of 10602-MM01 was blocked by the preincubation of the purified antibody with molar excess of recombinant human TNFa (5 μg, Cat. No. 10602-HNAE) for 1 hour (dashed line hisgram), then stained with a FITC-conjugated second step antibody. Right panel Human PBMC were stimulated for 4 hrs with Leukocyte Activation Cocktail (with BD GolgiPlug™, BD Pharmingen™ Cat. No. 550583), then cells were harvested and treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714).
Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.