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TNF-alpha / TNFA / TNFSF2 Antibody (FITC), Rabbit MAb

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Human TNFa Antibody Product Information
Immunogen:Recombinant Human TNF-alpha protein (Catalog#10602-HNAE)
Clone ID:101
Ig Type:Rabbit IgG
Concentration:10 μl/Test, 0.1 mg/ml
Endotoxin:
Formulation:Aqueous solution containing 0.5% BSA and 0.1% sodium azide
Preparation:This antibody was obtained from a rabbit immunized with purified, recombinant Human TNF-alpha (rh TNF-alpha; Catalog#10602-HNAE; NP_000585.2; Val 77-Leu 233) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Human TNFa Antibody Usage Guide
Specificity:Human TNF-alpha
Application:FCM
Storage:This antibody is stable for 12 months from date of receipt when stored at 2℃-8℃. Protected from prolonged exposure to light. Do not freeze !
Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Human TNFa Antibody FC Application Image
[Click to enlarge image]
Caption:
Flow cytometric analysis of anti-human TNFα on stimulated PBMC. Human peripheral blood mononuclear cells were stimulated for 6 hrs with Leukocyte Activation Cocktail, with BD GolgiPlug™ (BD Pharmingen™ Cat. No.550583). Cells were then fixed and permeabilized according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), followed by staining with Alexa Fluor® 647 Mouse Anti-Human CD8 (clone RPA-T8, BD Pharmingen™ Cat. No. 557708) and FITC Rabbit anti-Human TNFα. The dot plots were derived from gated lymphocytes.
TNF-alpha / TNFA Antibody (FITC), Rabbit MAb, Flow cytometric
Other TNFa Antibody Products
TNF-alpha/TNFA Background

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

TNF-alpha Protein & Antibody

Human TNF-alpha/TNFA References
  • Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
  • Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
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    Size / Price
    Catalog: 10602-R101-F-50
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    AvailabilityIn Stock
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    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"