Anti-TNF-alpha Antibody (FITC)

Cat: 10602-R101-F
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All TNF-alpha Reagents
Anti-TNF-alpha Antibody (FITC) (Rabbit Monoclonal antibody) General Information
Product name
Anti-TNF-alpha Antibody (FITC)
Validated applications
FCM
Species reactivity
Reacts with: Human
Specificity
Human TNF-alpha
Immunogen
Recombinant Human TNF-alpha protein (Catalog#10602-HNAE)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human TNF-alpha (rh TNF-alpha; Catalog#10602-HNAE; NP_000585.2; Val 77-Leu 233) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Source
Monoclonal Rabbit IgG Clone #101
Purification
Protein A
Formulation
Aqueous solution containing 0.5% BSA and 0.1% sodium azide
Conjugate
FITC
Concentration
10 μl/Test, 0.1 mg/ml
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Anti-TNF-alpha Antibody (FITC) (Rabbit Monoclonal antibody) Validated Applications

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-TNF-alpha Antibody (FITC) (Rabbit Monoclonal antibody) Images
Human TNF-alpha/TNFA/TNFSF2 Flow Cytometry (FC) 5383
Flow cytometric analysis of anti-human TNFα on stimulated PBMC. Human peripheral blood mononuclear cells were stimulated for 6 hrs with Leukocyte Activation Cocktail, with BD GolgiPlug™ (BD Pharmingen™ Cat. No.550583). Cells were then fixed and permeabilized according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), followed by staining with Alexa Fluor® 647 Mouse Anti-Human CD8 (clone RPA-T8, BD Pharmingen™ Cat. No. 557708) and FITC Rabbit anti-Human TNFα. The dot plots were derived from gated lymphocytes.
Anti-TNF-alpha Antibody (FITC) Alternative Names
Anti-DIF Antibody;Anti-TNF-alpha Antibody;Anti-TNFA Antibody;Anti-TNFSF2 Antibody
TNF-alpha Background Information

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

TNF-alpha Protein & Antibody

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
tumor necrosis factor
Research Areas
References
  • Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
  • Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-TNF-alpha Antibody Rabbit Monoclonal ELISA(Cap) 60002-R303
    Anti-TNF-alpha Antibody Mouse Monoclonal ELISA,ELISA(Det) 60002-MM11
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 70003-RP01
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 60002-RP01
    Anti-TNF-alpha Antibody Rabbit Monoclonal ELISA 60002-R030
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 70003-RP02
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 90330-RP01
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 60002-T16
    Anti-TNF-alpha Antibody Rabbit Monoclonal ELISA 70003-R001
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 80045-RP02
    Anti-TNF-alpha Antibody Rabbit Polyclonal WB,ELISA,ICC/IF,IF 80045-T50
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 80045-T16
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 80045-RP01
    Anti-TNF-alpha Antibody Rabbit Monoclonal ELISA 50349-R009
    Anti-TNF-alpha Antibody Rabbit Monoclonal ELISA(Cap) 50349-R023
    TNF-alpha/TNFA/TNFSF2 Neutralizing Antibody Rabbit Monoclonal Neutralization 50349-RN023
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 50349-RP02
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 50349-T16
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 50349-RP01
    Anti-TNF-alpha Antibody Rabbit Monoclonal ELISA 10602-RE23
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 10602-T16
    Anti-TNF-alpha Antibody Mouse Monoclonal ELISA,ELISA(Cap),FCM 10602-MM01
    Anti-TNF-alpha Antibody Rabbit Polyclonal WB,ELISA 10602-RP03
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 10602-RP01
    Anti-TNF-alpha Antibody (FITC) Mouse Monoclonal FCM 10602-MM01-F
    Anti-TNF-alpha Antibody (PE) Rabbit Monoclonal FCM 10602-R101-P
    Anti-TNF-alpha Antibody (APC) Rabbit Monoclonal FCM 10602-R101-A
    TNF-alpha / TNFA Neutralizing Antibody Mouse Monoclonal Neutralization 10602-MM0N1
    Anti-TNF-alpha Antibody Rabbit Polyclonal WB,ELISA,IP 10602-RP02
    TNF-alpha / TNFA / TNFSF2 Neutralizing Antibody Rabbit Monoclonal FCM,Neutralization 10602-R10N1
    Anti-TNF-alpha Antibody Rabbit Monoclonal ELISA 10602-R211
    Anti-TNF-alpha Antibody Mouse Monoclonal ELISA,ELISA(Det) 10602-MM08
    Anti-TNF-alpha Antibody Rabbit Monoclonal FCM 10602-R101
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