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TNF-alpha / TNFA / TNFSF2 Antibody (FITC), Mouse MAb

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Human TNFa Antibody Product Information
Immunogen:Recombinant Human TNF-alpha protein (Catalog#10602-HNAE)
Clone ID:01
Ig Type:Mouse IgG1
Concentration:10 μl/Test, 0.1 mg/ml
Endotoxin:
Formulation:Aqueous solution containing 0.5% BSA and 0.1% sodium azide
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human TNF-alpha (rh TNF-alpha; Catalog#10602-HNAE; NP_000585.2; Val 77-Leu 233) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Human TNFa Antibody Usage Guide
Specificity:Human TNF-alpha
Application:FCM
Storage:This antibody is stable for 12 months from date of receipt when stored at 2℃-8℃. Protected from prolonged exposure to light. Do not freeze !
Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Human TNFa Antibody FC Application Image
TNF-alpha / TNFA Antibody (FITC), Mouse MAb, Flow cytometric
[Click to enlarge image]
Caption:
Flow cytometric analysis of anti-human TNFα on stimulated PBMC. Human peripheral blood mononuclear cells were stimulated for 6 hrs with Leukocyte Activation Cocktail, with BD GolgiPlug™ (BD Pharmingen™ Cat. No.550583). Cells were then fixed and permeabilized according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), followed by staining with Alexa Fluor® 647 Mouse Anti-Human CD8 (clone RPA-T8, BD Pharmingen™ Cat. No. 557708) and FITC Mouse anti-Human TNFα. The dot plots were derived from gated lymphocytes.
Other TNFa Antibody Products
Reactivity: Human  
Application: B/N  
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10602-R10N1-200
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Reactivity: Human  
Application: ELISA  WB  IP  
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10602-RP02-50
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Reactivity: Human  
Application: FCM  
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10602-R101-A-100
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Reactivity: Human  
Application: B/N  
  • Slide 1
10602-MM0N1-500
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Reactivity: Human  
Application: ELISA  
    10602-RP01-100
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    Reactivity: Human  
    Application: ELISA  FCM  
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    10602-MM01-50
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    Reactivity: Human  
    Application: ELISA  WB  
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    10602-RP03-100
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    100 µg 
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    Reactivity: Human  
    Application: ELISA  
      10602-R211-50
      10602-R211-100
      50 µg 
      100 µg 
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      Reactivity: Human  
      Application: FCM  
      • Slide 1
      10602-R101-F-50
      10602-R101-F-100
      50 Tests 
      100 Tests 
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      Reactivity: Human  
      Application: FCM  
      • Slide 1
      10602-R101-P-100
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      100 Tests 
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      Reactivity: Human  
      Application: ELISA  
        10602-T16-100
        10602-T16-50
        10602-T16-200
        100 µg 
        50 µg 
        200 µg 
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        Reactivity: Human  
        Application: ELISA  
          10602-RE23-50
          10602-RE23-100
          50 µg 
          100 µg 
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          Reactivity: Mouse  
          Application: ELISA  
            50349-RP01-400
            50349-RP01-100
            50349-RP01-200
             
            100 µg 
            200 µg 
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            Reactivity: Mouse  
            Application: ELISA  
              50349-RP02-200
              50349-RP02-50
              50349-RP02-100
              200 µg 
              50 µg 
              100 µg 
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              Reactivity: Mouse  
              Application: ELISA  
                50349-R009-50
                50349-R009-100
                50 µg 
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                Reactivity: Rat  
                Application: ELISA  
                  80045-RP01-200
                  80045-RP01-100
                  80045-RP01-400
                  200 µg 
                  100 µg 
                   
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                  Reactivity: Rat  
                  Application: ELISA  
                    80045-RP02-50
                    80045-RP02-100
                    80045-RP02-200
                    50 µg 
                    100 µg 
                    200 µg 
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                    Reactivity: Cynomolgus  
                    Application: ELISA  
                      90330-RP01-400
                      90330-RP01-200
                      90330-RP01-100
                       
                      200 µg 
                      100 µg 
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                      Reactivity: Canine  
                      Application: ELISA  
                        70003-R001-50
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                        50 µg 
                        100 µg 
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                        Reactivity: Canine  
                        Application: ELISA  
                          70003-RP01-400
                          70003-RP01-100
                          70003-RP01-200
                           
                          100 µg 
                          200 µg 
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                          Reactivity: Canine  
                          Application: ELISA  
                            70003-RP02-200
                            70003-RP02-50
                            70003-RP02-100
                            200 µg 
                            50 µg 
                            100 µg 
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                            Reactivity: Canine  
                            Application: ELISA  
                              70003-MM04-200
                              70003-MM04-50
                              70003-MM04-100
                              200 µg 
                              50 µg 
                              100 µg 
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                              Reactivity: Ferret  
                              Application: ELISA  
                                60002-T16-100
                                60002-T16-50
                                60002-T16-200
                                100 µg 
                                50 µg 
                                200 µg 
                                Add to Cart
                                Reactivity: Ferret  
                                Application: ELISA  
                                  60002-R030-50
                                  60002-R030-100
                                  50 µg 
                                  100 µg 
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                                  Reactivity: Ferret  
                                  Application: ELISA  
                                    60002-MM11-200
                                    60002-MM11-50
                                    60002-MM11-100
                                    200 µg 
                                    50 µg 
                                    100 µg 
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                                    Reactivity: Ferret  
                                    Application: ELISA  
                                      60002-RP01-100
                                      60002-RP01-200
                                      60002-RP01-400
                                      100 µg 
                                      200 µg 
                                       
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                                      TNF-alpha/TNFA/TNFSF2 Background

                                      Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

                                      TNF-alpha Protein & Antibody

                                      Human TNF-alpha/TNFA/TNFSF2 References
                                    • Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
                                    • Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
                                    • Product nameProduct name
                                      Size / Price
                                      Catalog: 10602-MM01-F-50
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                                      Size:
                                      50 Tests
                                      100 Tests
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                                      All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
                                      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"