|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human TNFα ORF mammalian expression plasmid, C-GFPSpark tag||HG10602-ACG|
|Human TNFα ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10602-ACR|
|Human TNFα ORF mammalian expression plasmid, C-Flag tag||HG10602-CF|
|Human TNFα ORF mammalian expression plasmid, C-His tag||HG10602-CH|
|Human TNFα ORF mammalian expression plasmid, C-Myc tag||HG10602-CM|
|Human TNFα ORF mammalian expression plasmid, C-HA tag||HG10602-CY|
|Human TNFα ORF mammalian expression plasmid, N-Flag tag||HG10602-NF|
|Human TNFα ORF mammalian expression plasmid, N-His tag||HG10602-NH|
|Human TNFα ORF mammalian expression plasmid, N-Myc tag||HG10602-NM|
|Human TNFα ORF mammalian expression plasmid, N-HA tag||HG10602-NY|
|Human TNFα natural ORF mammalian expression plasmid||HG10602-UT|
|Learn more about expression Vectors|
Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.