Human TMIGD2 transcript variant 1 Gene cDNA Clone / ORF Clone

TMIGD2 cDNA Clone Datasheet

TMIGD2 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_144615.2
• cDNA Size: 849
• Gene Synonym: TMIGD2
• Source: Human

TMIGD2 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens transmembrane and immunoglobulin domain containing 2, transcript variant 1 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 710 C/G resulting in the amino acid Ala substitution by Gly.

TMIGD2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TMIGD2 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

TMIGD2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGGTCCC CGGGCATGGT GCTGGGCCTC CTGGTGCAGA TCTGGGCCCT GCAAGAAGCC
61       TCAAGCCTGA GCGTGCAGCA GGGGCCCAAC TTGCTGCAGG TGAGGCAGGG CAGTCAGGCG
121      ACCCTGGTCT GCCAGGTGGA CCAGGCCACA GCCTGGGAAC GGCTCCGTGT TAAGTGGACA
181      AAGGATGGGG CCATCCTGTG TCAACCGTAC ATCACCAACG GCAGCCTCAG CCTGGGGGTC
241      TGCGGGCCCC AGGGACGGCT CTCCTGGCAG GCACCCAGCC ATCTCACCCT GCAGCTGGAC
301      CCTGTGAGCC TCAACCACAG CGGGGCGTAC GTGTGCTGGG CGGCCGTAGA GATTCCTGAG
361      TTGGAGGAGG CTGAGGGCAA CATAACAAGG CTCTTTGTGG ACCCAGATGA CCCCACACAG
421      AACAGAAACC GGATCGCAAG CTTCCCAGGA TTCCTCTTCG TGCTGCTGGG GGTGGGAAGC
481      ATGGGTGTGG CTGCGATCGT GTGGGGTGCC TGGTTCTGGG GCCGCCGCAG CTGCCAGCAA
541      AGGGACTCAG GTAACAGCCC AGGAAATGCA TTCTACAGCA ACGTCCTATA CCGGCCCCGG
601      GGGGCCCCAA AGAAGAGTGA GGACTGCTCT GGAGAGGGGA AGGACCAGAG GGGCCAGAGC
661      ATTTATTCAA CCTCCTTCCC GCAACCGGCC CCCCGCCAGC CGCACCTGGG GTCAAGACCC
721      TGCCCCAGCC CGAGACCCTG CCCCAGCCCC AGGCCCGGCC ACCCCGTCTC TATGGTCAGG
781      GTCTCTCCTA GACCAAGCCC CACCCAGCAG CCGAGGCCAA AAGGGTTCCC CAAAGTGGGA
841      GAGGAGTGA

 

Quality Control:cDNA sequencing results is available upon request.