Human TMEM27 Gene cDNA Clone / ORF Clone

TMEM27 cDNA Clone Datasheet

TMEM27 cDNA Clone Product Information

• Gene Bank Ref.ID: BC014317
• cDNA Size: 669
• Gene Synonym: NX17; NX-17; TMEM27
• Source: Human

TMEM27 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens transmembrane protein 27 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

TMEM27 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TMEM27 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

TMEM27 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTTGTGGC TGCTCTTTTT TCTGGTGACT GCCATTCATG CTGAACTCTG TCAACCAGGT
61       GCAGAAAATG CTTTTAAAGT GAGACTTAGT ATCAGAACAG CTCTGGGAGA TAAAGCATAT
121      GCCTGGGATA CCAATGAAGA ATACCTCTTC AAAGCGATGG TAGCTTTCTC CATGAGAAAA
181      GTTCCCAACA GAGAAGCAAC AGAAATTTCC CATGTCCTAC TTTGCAATGT AACCCAGAGG
241      GTATCATTCT GGTTTGTGGT TACAGACCCT TCAAAAAATC ACACCCTTCC TGCTGTTGAG
301      GTGCAATCAG CCATAAGAAT GAACAAGAAC CGGATCAACA ATGCCTTCTT TCTAAATGAC
361      CAAACTCTGG AATTTTTAAA AATCCCTTCC ACACTTGCAC CACCCATGGA CCCATCTGTG
421      CCCATCTGGA TTATTATATT TGGTGTGATA TTTTGCATCA TCATAGTTGC AATTGCACTA
481      CTGATTTTAT CAGGGATCTG GCAACGTAGA AGAAAGAACA AAGAACCATC TGAAGTGGAT
541      GACGCTGAAG ATAAGTGTGA AAACATGATC ACAATTGAAA ATGGCATCCC CTCTGATCCC
601      CTGGACATGA AGGGAGGGCA TATTAATGAT GCCTTCATGA CAGAGGATGA GAGGCTCACC
661      CCTCTCTGA

 

Quality Control:cDNA sequencing results is available upon request.