TMEM27 Protein

Transmembrane protein 27 Protein Datasheet

TMEM27 Protein Product Information

• Synonym: TMEM27
• Protein Construction: A DNA sequence encoding the human TMEM27(Q9HBJ8) (Met1-Pro141) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
• Source: Human
• Expression Host: Human Cells

TMEM27 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu 15
• Molecular Mass: The recombinant human TMEM27 /Fc is a disulfide-linked homodimer. The reduced monomer comprises 368 amino acids and has a predicted molecular mass of 41.4 kDa. The apparent molecular mass of the protein is approximately 53-57 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: TMEM27 protein

TMEM27 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

TMEM27 Protein Description

TMEM27 is a membrane protein. It forms dimers and its extracellular domain is glycosylated, cleaved and shed from the plasma membrane of beta cells. Thus it has been proposed as a beta cell mass biomarker. Overexpression of TMEM27 leads to increased thymidine incorporation, whereas silencing of Tmem27 using RNAi results in a reduction of cell replication. Furthermore, transgenic mice with increased expression of Tmem27 in pancreatic beta cells exhibit increased beta cell mass. TMEM27 is important for trafficking amino acid transporters to the apical brush border of proximal tubules.

References

1. Pasquali L. et al., 2009, J Genet. 88 (1): 105-8.
2. Tosetto E. et al., 2009, Pediatr Nephrol. 24 (10): 1967-73.
3. Singer D. et al., 2011, Channels (Austin). 5 (5): 410-23.