Human TMEM25 Gene cDNA Clone / ORF Clone

TMEM25 cDNA Clone Datasheet

TMEM25 cDNA Clone Product Information

• Gene Bank Ref.ID: BC051841
• cDNA Size: 969
• Gene Synonym: UNQ2531/PRO6030; FLJ14399; TMEM25
• Source: Human

TMEM25 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens transmembrane protein 25 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

TMEM25 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TMEM25 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

TMEM25 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCGCTGC CTCCAGGCCC AGCCGCCCTC CGGCACACAC TGCTGCTCCT GCCAGCCCTT
61       CTGAGCTCAG GTTGGGGGGA GTTGGAGCCA CAAATAGATG GTCAGACCTG GGCTGAGCGG
121      GCACTTCGGG AGAATGAACG CCACGCCTTC ACCTGCCGGG TGGCAGGGGG GCCTGGCACC
181      CCCAGATTGG CCTGGTATCT GGATGGACAG CTGCAGGAGG CCAGCACCTC AAGACTGCTG
241      AGCGTGGGAG GGGAGGCCTT CTCTGGAGGC ACCAGCACCT TCACTGTCAC TGCCCATCGG
301      GCCCAGCATG AGCTCAACTG CTCTCTGCAG GACCCCAGAA GTGGCCGATC AGCCAACGCC
361      TCTGTCATCC TTAATGTGCA ATTCAAGCCA GAGATTGCCC AAGTCGGCGC CAAGTACCAG
421      GAAGCTCAGG GCCCAGGCCT CCTGGTTGTC CTGTTTGCCC TGGTGCGTGC CAACCCGCCG
481      GCCAATGTCA CCTGGATCGA CCAGGATGGG CCAGTGACTG TCAACACCTC TGACTTCCTG
541      GTGCTGGATG CGCAGAACTA CCCCTGGCTC ACCAACCACA CGGTGCAGCT GCAGCTCCGC
601      AGCCTGGCAC ACAACCTCTC GGTGGTGGCC ACCAATGACG TGGGTGTCAC CAGTGCGTCG
661      CTTCCAGCCC CAGGCCCCTC CCGGCACCCA TCTCTGATAT CAAGTGACTC CAACAACCTA
721      AAACTCAACA ACGTGCGCCT GCCACGGGAG AACATGTCCC TCCCGTCCAA CCTTCAGCTC
781      AATGACCTCA CTCCAGATTC CAGAGCAGTG AAACCAGCAG ACCGGCAGAT GGCTCAGAAC
841      AACAGCCGGC CAGAGCTTCT GGACCCGGAG CCCGGCGGCC TCCTCACCAG CCGAGGTTTC
901      ATCCGCCTCC CAGTGCTGGG CTATATCTAT CGAGTGTCCA GCGTGAGCAG TGATGAGATC
961      TGGCTCTGA

 

Quality Control:cDNA sequencing results is available upon request.