Human TMEM132A Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13418-G	Cloning vector ($295) pCMV / hygro ($545) pCMV / hygro - HA ($545) pCMV / hygro - His ($545) pCMV / hygro - Myc ($545) pCMV / hygro - FLAG ($545)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

TMEM132A cDNA Clone Datasheet

TMEM132A cDNA Clone Product Information

Gene Bank Ref.ID:	NM_178031.2
cDNA Size:	3072
Gene Synonym:	GBP; FLJ20539; HSPA5BP1; MGC138669; DKFZp547E212; TMEM132A
Source:	Human

TMEM132A cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens transmembrane protein 132A DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutations: 2470 G/A resulting in the amino acid Glu substitution by Lys and 504 C/A not causing the amino acid variation.

TMEM132A cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TMEM132A cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

TMEM132A cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTGCGCGC GGATGGCCGG TCGCACAACA GCGGCCCCTC GGGGGCCCTA CGGCCCCTGG
61       CTCTGCCTCC TGGTGGCCCT CGCCCTGGAC GTCGTGAGAG TGGACTGTGG CCAGGCTCCC
121      CTGGACCCTG TCTACCTGCC GGCAGCCCTG GAGCTCCTAG ACGCCCCTGA ACACTTCCGT
181      GTGCAGCAGG TGGGCCACTA CCCACCTGCC AACTCCTCTC TGAGCTCCCG ATCTGAGACC
241      TTTCTGCTCC TACAGCCCTG GCCCAGGGCC CAGCCACTTC TCCGGGCCTC CTACCCACCT
301      TTTGCCACTC AGCAGGTGGT CCCCCCTCGA GTCACTGAGC CCCACCAACG GCCAGTCCCA
361      TGGGACGTGC GGGCCGTTTC AGTGGAAGCG GCTGTGACTC CAGCAGAGCC CTACGCCCGG
421      GTTCTCTTCC ACCTCAAAGG GCAGGATTGG CCACCAGGGT CTGGCAGCCT GCCCTGTGCC
481      CGGCTCCATG CCACACACCC TGCAGGCACT GCTCACCAAG CCTGCCGCTT CCAGCCATCC
541      CTGGGCGCCT GCGTGGTGGA GCTGGAGCTT CCCTCGCACT GGTTCTCACA GGCCTCCACC
601      ACACGGGCCG AGCTGGCCTA CACGCTTGAG CCTGCAGCTG AGGGCCCTGG GGGCTGTGGC
661      TCCGGCGAGG AGAACGACCC TGGGGAGCAG GCCCTCCCAG TGGGGGGTGT GGAGCTGCGC
721      CCAGCAGACC CCCCGCAGTA CCAGGAGGTA CCTCTGGACG AGGCTGTGAC TCTGCGGGTG
781      CCTGACATGC CAGTGCGGCC CGGCCAGCTC TTTAGTGCTA CCCTCCTGCT TCGGCACAAC
841      TTCACAGCCA GCCTCCTGAC CCTGCGGATC AAGGTGAAGA AGGGGCTGCA TGTGACAGCC
901      GCCCGCCCAG CCCAGCCCAC ACTCTGGACT GCCAAGCTGG ACCGCTTCAA GGGCTCCAGG
961      CACCACACCA CCCTCATCAC CTGCCACCGT GCTGGGCTCA CAGAGCCAGA TTCCAGTCCC
1021     CTTGAACTGT CTGAGTTCCT ATGGGTGGAC TTTGTGGTGG AGAATAGCAC TGGTGGGGGC
1081     GTAGCGGTCA CTCGCCCCGT CACGTGGCAG CTGGAGTACC CAGGCCAGGC CCCTGAAGCA
1141     GAGAAGGACA AAATGGTGTG GGAAATCCTG GTGTCTGAGC GGGACATCAG AGCCCTTATC
1201     CCACTGGCCA AGGCTGAGGA GCTGGTGAAT ACAGCACCAC TGACTGGAGT GCCCCAGCAT
1261     GTCCCCGTGC GCCTTGTCAC TGTGGACGGC GGGGGGGCCT TGGTGGAGGT GACAGAGCAT
1321     GTCGGCTGCG AGTCTGCCAA CACACAGGTC CTGCAGGTGT CTGAGGCCTG TGATGCCGTG
1381     TTCGTGGCTG GCAAGGAGAG CCGGGGCGCC CGGGGGGTGC GAGTGGACTT CTGGTGGCGC
1441     CGGCTCCGCG CCTCGCTGCG GCTGACCGTG TGGGCCCCCC TGCTACCGCT GCGTATCGAG
1501     CTCACCGACA CCACCCTCGA GCAGGTCCGC GGCTGGAGGG TACCTGGCCC TGCTGAAGGG
1561     CCTGCGGAAC CCGCTGCAGA GGCGTCGGAT GAGGCCGAGC GGCGCGCCCG TGGCTGCCAC
1621     CTGCAGTACC AGCGGGCCGG TGTGCGCTTC CTCGCCCCCT TCGCGGCCCA CCCGCTGGAC
1681     GGCGGCCGCC GCCTCACGCA CCTGCTTGGC CCCGACTGGC TGCTAGACGT GTCCCACCTC
1741     GTGGCGCCAC ACGCCCGCGT GCTGGACTCG CGTGTAGCCT CTCTGGAGGG TGGCCGTGTC
1801     GTGGTGGGCC GGGAGCCCGG TGTCACCTCC ATTGAGGTGC GTTCCCCACT GTCTGACTCC
1861     ATCCTGGGGG AGCAGGCGCT GGCTGTGACG GACGACAAGG TCTCAGTGCT GGAGCTGAGG
1921     GTGCAGCCAG TGATGGGCAT CTCGCTGACC TTGAGCCGGG GCACTGCCCA CCCCGGGGAG
1981     GTCACAGCTA CGTGCTGGGC ACAGTCAGCC CTTCCCGCCC CAAAGCAGGA GGTGGCCCTC
2041     TCCCTATGGC TGTCCTTCTC TGATCACACT GTGGCCCCAG CTGAGCTCTA CGACCGCCGT
2101     GACCTGGGAC TGTCCGTCTC AGCCGAGGAG CCTGGTGCCA TCCTGCCAGC TGAGGAGCAG
2161     GGTGCCCAGC TCGGGGTGGT GGTGAGTGGG GCAGGCGCCG AGGGGCTGCC GCTGCATGTG
2221     GCTCTGCACC CGCCCGAGCC CTGCCGCCGG GGCCGCCACC GTGTGCCTCT GGCCTCTGGC
2281     ACCGCCTGGC TGGGGCTGCC CCCTGCCTCC ACTCCAGCCC CTGCTCTCCC ATCCAGCCCT
2341     GCTTGGAGCC CACCAGCCAC AGAAGCCACC ATGGGTGGTA AACGGCAGGT GGCAGGCAGT
2401     GTCGGGGGCA ACACAGGTGT GAGGGGCAAG TTTGAGCGGG CAGAGGAGGA GGCCAGGAAG
2461     GAGGAGACCA AAGCCAGGGA GGAGGAGGAG GAAGAGGAGG AGGAGATGGT CCCTGCCCCT
2521     CAGCATGTCA CTGAGCTAGA GCTGGGCATG TACGCCCTGC TGGGAGTCTT CTGCGTGGCC
2581     ATCTTCATCT TCTTGGTCAA TGGTGTGGTC TTCGTCCTGC GCTATCAGCG CAAAGAACCT
2641     CCCGACAGTG CCACTGACCC CACCTCCCCC CAGCCCCACA ACTGGGTCTG GCTGGGCACT
2701     GACCAGGAGG AACTGAGCCG CCAGCTGGAC CGGCAGTCCC CTGGCCCGCC CAAGGGGGAG
2761     GGGAGCTGCC CCTGTGAGAG TGGGGGAGGA GGGGAGGCCC CTACCCTGGC CCCTGGCCCT
2821     CCTGGGGGCA CCACCAGCTC CTCAAGCACC CTGGCCCGAA AGGAGGCTGG GGGGCGGCGG
2881     AAGCGAGTAG AGTTTGTGAC ATTTGCGCCA GCCCCTCCAG CCCAGTCACC TGAGGAGCCT
2941     GTAGGGGCCC CTGCTGTGCA GTCCATCCTT GTGGCAGGCG AGGAGGACAT CCGCTGGGTG
3001     TGTGAGGACA TGGGGCTGAA GGACCCTGAG GAGCTTCGCA ACTACATGGA GAGGATCCGG
3061     GGCAGCTCCT GA

Quality Control:cDNA sequencing results is available upon request.