Human TMED4 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13729-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

TMED4 cDNA Clone Datasheet

TMED4 cDNA Clone Product Information

Gene Bank Ref.ID:	BC057851
cDNA Size:	684
Gene Synonym:	HNLF; ERS25; TMED4
Source:	Human

TMED4 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens transmembrane emp24 protein transport domain conta DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 342 G/A not causing the amino acid variation.

TMED4 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TMED4 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

TMED4 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCAGGTG TCGGGGCTGG GCCTCTGCGG GCGATGGGGC GGCAGGCCCT GCTGCTTCTC
61       GCGCTGTGCG CCACAGGCGC CCAGGGGCTC TACTTCCACA TCGGCGAGAC CGAGAAGCGC
121      TGTTTCATCG AGGAAATCCC CGACGAGACC ATGGTCATCG GCAACTATCG TACCCAGATG
181      TGGGATAAGC AGAAGGAGGT CTTCCTGCCC TCGACCCCTG GCCTGGGCAT GCACGTGGAA
241      GTGAAGGACC CCGACGGCAA GGTGGTGCTG TCCCGGCAGT ACGGCTCGGA GGGCCGCTTC
301      ACGTTCACCT CCCACACGCC CGGTGACCAT CAAATCTGTC TACACTCCAA TTCTACCAGG
361      ATGGCTCTCT TCGCTGGTGG CAAACTGCGG GTGCATCTCG ACATCCAGGT TGGGGAGCAT
421      GCCAACAACT ACCCTGAGAT TGCTGCAAAA GATAAGCTGA CGGAGCTACA GCTCCGCGCC
481      CGCCAGTTGC TTGATCAGGT GGAACAGATT CAGAAGGAGC AGGATTACCA AAGGTATCGT
541      GAAGAGCGCT TCCGACTGAC GAGCGAGAGC ACCAACCAGA GGGTCCTATG GTGGTCCATT
601      GCTCAGACTG TCATCCTCAT CCTCACTGGC ATCTGGCAGA TGCGTCACCT CAAGAGCTTC
661      TTTGAGGCCA AGAAGCTGGT GTAG

Quality Control:cDNA sequencing results is available upon request.