TMED1 Protein (His Tag)

Transmembrane emp24 protein transport domain containing 1 Protein Datasheet

TMED1 Protein Product Information

• Synonym: TMED1, IL1RL1L, IL1RL1LG
• Protein Construction: A DNA sequence encoding the human TMED1 (Q13445) (Met1-Asn194) was expressed with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: Human Cells

TMED1 Protein QC Testing

• Purity: > 90% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ala 24
• Molecular Mass: The recombinant human TMED1 consists of 182 amino acids and predicts a molecular mass of 20.6 KDa. It migrates as an approximately 28 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH 7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: TMED1 protein

TMED1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

TMED1 Protein Description

TMED1 is a member of the EMP24/GP25L family. It is widely expressed and contains 1 GOLD domain. TMED1 may play a role in vesicular protein trafficking, mainly in the early secretory pathway. It may act as a cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and may be involved in vesicle coat formation at the cytoplasmic side. TMED1 binds to its receptor IL1RL1 and results in the activation of DNA binding by nuclear factor NF-kappa-B or transcription from the IL8 promoter and most likely requires other proteins to elicit these activities. Dendritic cells from Peyer's patches (but not from spleen) express TMED1 in response to treatment with LPS.

References

1. Colland F. et al., 2004, Genome Res. 14 (7): 1324-32.
2. Gerhard DS. et al., 2005, Nature. 437 (7062): 1173-8.
3. Gerhard DS. et al., 2004, Genome Res. 14 (10B): 2121-7.