Rat TLR9 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80386-G	Cloning vector ($295) pCMV / hygro ($545) pCMV / hygro - HA ($545) pCMV / hygro - His ($545) pCMV / hygro - Myc ($545) pCMV / hygro - FLAG ($545)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

TLR9 cDNA Clone Datasheet

TLR9 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_198131.1
cDNA Size:	3099
Gene Synonym:	Tlr9
Source:	Rat

TLR9 cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus toll-like receptor 9 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutations: 882 T/G resulting in the amino acid Ser substitution by Arg and 144 G/C，306 T/C，1185 G/A not causing the amino acid variation.

TLR9 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TLR9 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

TLR9 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGTTCTCT GTAGCAGGAC CCTGCACCCC TTGTCTCTCC TGGTACAGGC TGCAGTGCTG
61       GCTGAGGCTC TGGCCCTGGG TACCCTGCCT GCCTTCCTAC CCTGTGAACT GAAGCCTCAT
121      GGCCTGGTAG ACTGCAACTG GCTCTTCCTG AAGTCTGTGC CTCACTTCTC TGCCGCAGAA
181      CCCCGTTCCA ACATCACCAG CCTTTCCTTG ATCGCCAACC GCATCCACCA CCTGCACAAC
241      CTCGACTTTG TCCACCTGCC CAACGTGCGA CAGCTGAACC TCAAGTGGAA CTGTCCGCCC
301      CCTGGCCTCA GCCCCTTGCA CTTCTCCTGC CGCATGACCA TTGAGCCCAA AACCTTCCTG
361      GCTATGCGCA TGCTGGAAGA GCTGAACCTG AGCTATAACG GTATCACCAC TGTGCCCCGC
421      CTGCCCAGCT CCCTGACGAA TCTGAGCCTA AGCCACACCA ACATCCTGGT ACTCGATGCC
481      AGCAGCCTTG CTGGCCTGCA CAGCCTGCGA GTTCTCTTCA TGGACGGGAA CTGCTACTAC
541      AAGAACCCCT GCAACGGGGC GGTGAACGTG ACCCCGGACG CCTTCCTGGG CTTGAGCAAC
601      CTCACCCACT TGTCCCTTAA GTATAACAAC CTCACAGAGG TGCCCCGCCA ACTGCCCCCC
661      AGCCTGGAGT ACCTCCTGCT GTCCTATAAC CTCATCGTCA AGCTGGGGCC CGAAGACCTA
721      GCCAACCTGA CCTCCCTTCG AGTGCTTGAT GTGGGTGGGA ATTGCCGTCG CTGTGATCAC
781      GCCCCCGACC TCTGTACAGA ATGCCGGCAG AAGTCCCTTG ATCTGCACCC TCAGACTTTC
841      CATCACCTGA GCCACCTTGA AGGCCTGGTG CTGAAGGACA GGTCTCTCCA CTCGCTGAAC
901      TCCAAGTGGT TCCAGGGTCT GGTGAACCTC TCGGTGCTGG ACCTAAGCGA GAACTTTCTC
961      TACGAGAGCA TCAACAAAAC CAGCGCCTTT CAGAACCTGA CCCGTCTGCG CAAGCTCGAC
1021     CTGTCCTTCA ATTACTGCAA GAAGGTATCG TTCGCCCGCC TCCACCTGGC AAGTTCCTTC
1081     AAGAGCCTGG TGTCGCTGCA GGAGCTGAAC ATGAACGGCA TCTTCTTCCG CTTGCTCAAC
1141     AAGAACACGC TCAGGTGGCT GGCTGGTCTG CCCAAGCTCC ACACACTGCA CCTTCAAATG
1201     AATTTCATCA ACCAGGCGCA GCTCAGCGTC TTTAGTACCT TCCGAGCCCT TCGCTTTGTG
1261     GACCTGTCCA ATAATCGCAT CAGCGGGCCT CCAACGCTGT CCAGAGTCGC CCCCGAAAAG
1321     GCAGACGAGG CGGAGAAGGG GGTTCCATGG CCTGCAAGTC TCACCCCAGC TCTCCCGAGC
1381     ACTCCCGTCT CAAAGAACTT CATGGTCAGG TGTAAGAACC TCAGATTCAC CATGGACCTG
1441     TCTCGGAACA ACCTGGTGAC TATCAAGCCA GAGATGTTCG TCAACCTCTC CCATCTCCAG
1501     TGTCTGAGCC TGAGCCACAA CTGCATCGCG CAGGCTGTCA ATGGCTCTCA GTTCCTGCCG
1561     CTGACCAACC TGAAGGTGCT GGACCTGTCC TATAACAAGC TGGACCTGTA CCATTCGAAA
1621     TCGTTCAGTG AGCTCCCACA GTTGCAGGCC CTGGACCTGA GCTACAACAG CCAGCCCTTC
1681     AGCATGCAGG GGATAGGCCA CAACTTCAGT TTTCTGGCCA ATCTGTCCAG GTTACAGAAC
1741     CTTAGCCTGG CACACAATGA CATTCACAGC CGCGTGTCCT CACGCCTCTA CAGCACCTCA
1801     GTGGAGTATC TGGACTTCAG CGGCAACGGT GTGGGCCGCA TGTGGGACGA GGAGGACCTT
1861     TACCTCTATT TCTTCCAAGA CCTGAGAAGC CTGATTCATC TGGACCTATC TCAGAATAAG
1921     CTGCACATCC TCCGGCCCCA GAACCTCAAC TACCTCCCCA AGAGCCTGAC GAAGCTGAGT
1981     TTCCGTGACA ATCACCTCTC TTTCTTTAAC TGGAGCAGTC TGGCCTTCCT GCCCAATCTG
2041     CGAGACCTGG ACCTGGCAGG CAATCTACTA AAGGCCCTGA CCAACGGCAC CCTGCCTAAT
2101     GGCACGCTCC TCCAGAAACT GGATGTCAGT AGCAACAGTA TCGTCTTTGT GGTCCCAGCC
2161     TTCTTTGCTC TGGCGGTAGA GCTAAAAGAG GTCAACCTCA GCCATAACAT CCTCAAGACT
2221     GTGGATCGCT CCTGGTTTGG GCCCATTGTG ATGAACCTGA CGGTTCTAGA CGTGAGCAGC
2281     AACCCTCTGC ATTGTGCCTG CGGTGCACCC TTTGTAGACT TACTGCTGGA AGTGCAGACC
2341     AAGGTGCCTG GCCTGGCTAA CGGTGTGAAG TGTGGCAGTC CCCGCCAGCT GCAGGGCCGC
2401     AGCATCTTTG CGCAAGACCT GCGGCTGTGC CTGGATGACG TCCTTTCTCG GGACTGCTTT
2461     GGCCTTTCAC TCCTGGCTGT GGCCGTGGGC ACGGTGTTGC CTTTACTGCA GCATCTCTGC
2521     GGCTGGGACG TCTGGTACTG TTTCCATCTG TGCCTGGCAT GGCTACCTTT GCTGACCCGT
2581     GGCCGGCGCA GCGCCCAAGC TCTCCCTTAT GATGCCTTCG TGGTGTTCGA TAAGGCGCAG
2641     AGCGCGGTTG CTGACTGGGT GTATAACGAG CTTCGAGTGC GGCTAGAGGA GCGGCGCGGT
2701     CGCCGAGCCC TACGCTTGTG TCTGGAGGAC CGAGATTGGC TGCCTGGCCA GACACTCTTC
2761     GAGAACCTCT GGGCCTCCAT CTATGGCAGC CGCAAGACTC TGTTTGTGCT GGCCCACACG
2821     GACAAGGTCA GTGGCCTCCT GCGCACCAGC TTCCTGCTGG CTCAGCAGCG CCTGCTGGAG
2881     GACCGCAAGG ACGTGGTGGT GTTGGTGATC CTGCGCCCTG ATGCCCACCG CTCCCGCTAC
2941     GTGCGACTGC GCCAGCGCCT CTGCCGCCAG AGTGTGCTCT TCTGGCCCCA TCAGCCCAAC
3001     GGGCAGGGCA GCTTCTGGGC CCAGCTGAGT ACAGCCCTGA CTAGGGACAA CCACCACTTC
3061     TATAACCGGA ACTTCTGCCG GGGACCTACA GCAGAATAG

Quality Control:cDNA sequencing results is available upon request.