Human TLR4 transcript variant 1 ORF Expression Clone, Myc-tagged

TLR4 transcript variant 1 cDNA Clone Datasheet

TLR4 transcript variant 1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_138554.2
• cDNA Size: 2550
• Gene Synonym: TOLL, CD284, hToll, ARMD10
• Source: Human

TLR4 transcript variant 1 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens toll-like receptor 4, transcript variant 1 DNA.
• Vector: pCMV/hygro-Myc
• Myc Tag Sequence: CAGAAACTCATCTCAGAAGAGGATCTG
• Sequence Description: Identical with the Gene Bank Ref. ID sequence.

TLR4 transcript variant 1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TLR4 transcript variant 1 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

TLR4 transcript variant 1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGATGTCTG CCTCGCGCCT GGCTGGGACT CTGATCCCAG CCATGGCCTT CCTCTCCTGC
61       GTGAGACCAG AAAGCTGGGA GCCCTGCGTG GAGGTGGTTC CTAATATTAC TTATCAATGC
121      ATGGAGCTGA ATTTCTACAA AATCCCCGAC AACCTCCCCT TCTCAACCAA GAACCTGGAC
181      CTGAGCTTTA ATCCCCTGAG GCATTTAGGC AGCTATAGCT TCTTCAGTTT CCCAGAACTG
241      CAGGTGCTGG ATTTATCCAG GTGTGAAATC CAGACAATTG AAGATGGGGC ATATCAGAGC
301      CTAAGCCACC TCTCTACCTT AATATTGACA GGAAACCCCA TCCAGAGTTT AGCCCTGGGA
361      GCCTTTTCTG GACTATCAAG TTTACAGAAG CTGGTGGCTG TGGAGACAAA TCTAGCATCT
421      CTAGAGAACT TCCCCATTGG ACATCTCAAA ACTTTGAAAG AACTTAATGT GGCTCACAAT
481      CTTATCCAAT CTTTCAAATT ACCTGAGTAT TTTTCTAATC TGACCAATCT AGAGCACTTG
541      GACCTTTCCA GCAACAAGAT TCAAAGTATT TATTGCACAG ACTTGCGGGT TCTACATCAA
601      ATGCCCCTAC TCAATCTCTC TTTAGACCTG TCCCTGAACC CTATGAACTT TATCCAACCA
661      GGTGCATTTA AAGAAATTAG GCTTCATAAG CTGACTTTAA GAAATAATTT TGATAGTTTA
721      AATGTAATGA AAACTTGTAT TCAAGGTCTG GCTGGTTTAG AAGTCCATCG TTTGGTTCTG
781      GGAGAATTTA GAAATGAAGG AAACTTGGAA AAGTTTGACA AATCTGCTCT AGAGGGCCTG
841      TGCAATTTGA CCATTGAAGA ATTCCGATTA GCATACTTAG ACTACTACCT CGATGATATT
901      ATTGACTTAT TTAATTGTTT GACAAATGTT TCTTCATTTT CCCTGGTGAG TGTGACTATT
961      GAAAGGGTAA AAGACTTTTC TTATAATTTC GGATGGCAAC ATTTAGAATT AGTTAACTGT
1021     AAATTTGGAC AGTTTCCCAC ATTGAAACTC AAATCTCTCA AAAGGCTTAC TTTCACTTCC
1081     AACAAAGGTG GGAATGCTTT TTCAGAAGTT GATCTACCAA GCCTTGAGTT TCTAGATCTC
1141     AGTAGAAATG GCTTGAGTTT CAAAGGTTGC TGTTCTCAAA GTGATTTTGG GACAACCAGC
1201     CTAAAGTATT TAGATCTGAG CTTCAATGGT GTTATTACCA TGAGTTCAAA CTTCTTGGGC
1261     TTAGAACAAC TAGAACATCT GGATTTCCAG CATTCCAATT TGAAACAAAT GAGTGAGTTT
1321     TCAGTATTCC TATCACTCAG AAACCTCATT TACCTTGACA TTTCTCATAC TCACACCAGA
1381     GTTGCTTTCA ATGGCATCTT CAATGGCTTG TCCAGTCTCG AAGTCTTGAA AATGGCTGGC
1441     AATTCTTTCC AGGAAAACTT CCTTCCAGAT ATCTTCACAG AGCTGAGAAA CTTGACCTTC
1501     CTGGACCTCT CTCAGTGTCA ACTGGAGCAG TTGTCTCCAA CAGCATTTAA CTCACTCTCC
1561     AGTCTTCAGG TACTAAATAT GAGCCACAAC AACTTCTTTT CATTGGATAC GTTTCCTTAT
1621     AAGTGTCTGA ACTCCCTCCA GGTTCTTGAT TACAGTCTCA ATCACATAAT GACTTCCAAA
1681     AAACAGGAAC TACAGCATTT TCCAAGTAGT CTAGCTTTCT TAAATCTTAC TCAGAATGAC
1741     TTTGCTTGTA CTTGTGAACA CCAGAGTTTC CTGCAATGGA TCAAGGACCA GAGGCAGCTC
1801     TTGGTGGAAG TTGAACGAAT GGAATGTGCA ACACCTTCAG ATAAGCAGGG CATGCCTGTG
1861     CTGAGTTTGA ATATCACCTG TCAGATGAAT AAGACCATCA TTGGTGTGTC GGTCCTCAGT
1921     GTGCTTGTAG TATCTGTTGT AGCAGTTCTG GTCTATAAGT TCTATTTTCA CCTGATGCTT
1981     CTTGCTGGCT GCATAAAGTA TGGTAGAGGT GAAAACATCT ATGATGCCTT TGTTATCTAC
2041     TCAAGCCAGG ATGAGGACTG GGTAAGGAAT GAGCTAGTAA AGAATTTAGA AGAAGGGGTG
2101     CCTCCATTTC AGCTCTGCCT TCACTACAGA GACTTTATTC CCGGTGTGGC CATTGCTGCC
2161     AACATCATCC ATGAAGGTTT CCATAAAAGC CGAAAGGTGA TTGTTGTGGT GTCCCAGCAC
2221     TTCATCCAGA GCCGCTGGTG TATCTTTGAA TATGAGATTG CTCAGACCTG GCAGTTTCTG
2281     AGCAGTCGTG CTGGTATCAT CTTCATTGTC CTGCAGAAGG TGGAGAAGAC CCTGCTCAGG
2341     CAGCAGGTGG AGCTGTACCG CCTTCTCAGC AGGAACACTT ACCTGGAGTG GGAGGACAGT
2401     GTCCTGGGGC GGCACATCTT CTGGAGACGA CTCAGAAAAG CCCTGCTGGA TGGTAAATCA
2461     TGGAATCCAG AAGGAACAGT GGGTACAGGA TGCAATTGGC AGGAAGCAAC ATCTATCGCT1
2521     CAGAAACTCA TCTCAGAAGA GGATCTG2TAA

 

1. GCT was the nucleotide residue from the restriction site during plasmid construction,
which has no influence on protein expression.
2. Myc Tag

 

 

Quality Control:cDNA sequencing results is available upon request.