|Datasheet||Specific References||Reviews||Related Products||Protocols|
|ORF Clone of Rattus norvegicus toll-like receptor 4 DNA.|
|Identical with the Gene Bank Ref. ID sequence except for the point mutations: 220 A/T resulting in the amino acid Thr substitution by Ser and 309 C/T，498 G/A，978 G/A not causing the amino acid variation.|
|Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.|
|The Whatman FTA elute card can be stored at room temperature for three months under dry condition.|
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Rat TLR4 Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||RG80389-G-F|
|Rat TLR4 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||RG80389-G-H|
|Rat TLR4 Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||RG80389-G-M|
|Rat TLR4 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||RG80389-G-N|
|Rat TLR4 Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||RG80389-G-Y|
|Product name||Product name|
TLR4, also known as TLR-4, is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. TLR4 is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. TLR 4 has also been designated as CD284 (cluster of differentiation 284). It has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. TLR4 Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). It acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. It is also involved in LPS-independent inflammatory responses triggered by Ni(2+).