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Product Catalog
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Human TLR3 Gene cDNA Clone / ORF Clone
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| HG10190-M |
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YES | 
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General Information
Manufactured By: Sino Biological Inc.
Gene Bank Ref.ID: NM_003265.2
ORF Size: 2707
Gene Name Synonym: TLR3, CD283
Source: Human.
Description
ORF Description: ORF Clone of Homo sapiens toll-like receptor 3 DNA
Vector: Vector pMD18-T Simple
Sequence Description: Same as Gene Bank Ref. ID sequence with the nucleotide 1, 18, 2710-2715 deletion mutation and the point mutation 1234 C/T resulting in the amino acid Leu substitution by Phe.
Shipping
5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
Usage Protocol
- Remove the marked edge in the center of the spot .
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to a heating block at 95℃ for 15-30 min.
- At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
- Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .
ORF Sequence and Quality Control
Sequence:
1 TGAGACAGAC TTTGCCTGTA TCTACTTTTG GGGGGGCCTT TTGCCCTTTG GGATGCTGTG 61 TGCATCCTCC ACCACCAAGT GCACTGTTAG CCATGAAGTT GCTGACTGCA GCCACCTGAA 121 GTTGACTCAG GTACCCGATG ATCTACCCAC AAACATAACA GTGTTGAACC TTACCCATAA 181 TCAACTCAGA AGATTACCAG CCGCCAACTT CACAAGGTAT AGCCAGCTAA CTAGCTTGGA 241 TGTAGGATTT AACACCATCT CAAAACTGGA GCCAGAATTG TGCCAGAAAC TTCCCATGTT 301 AAAAGTTTTG AACCTCCAGC ACAATGAGCT ATCTCAACTT TCTGATAAAA CCTTTGCCTT 361 CTGCACGAAT TTGACTGAAC TCCATCTCAT GTCCAACTCA ATCCAGAAAA TTAAAAATAA 421 TCCCTTTGTC AAGCAGAAGA ATTTAATCAC ATTAGATCTG TCTCATAATG GCTTGTCATC 481 TACAAAATTA GGAACTCAGG TTCAGCTGGA AAATCTCCAA GAGCTTCTAT TATCAAACAA 541 TAAAATTCAA GCGCTAAAAA GTGAAGAACT GGATATCTTT GCCAATTCAT CTTTAAAAAA 601 ATTAGAGTTG TCATCGAATC AAATTAAAGA GTTTTCTCCA GGGTGTTTTC ACGCAATTGG 661 AAGATTATTT GGCCTCTTTC TGAACAATGT CCAGCTGGGT CCCAGCCTTA CAGAGAAGCT 721 ATGTTTGGAA TTAGCAAACA CAAGCATTCG GAATCTGTCT CTGAGTAACA GCCAGCTGTC 781 CACCACCAGC AATACAACTT TCTTGGGACT AAAGTGGACA AATCTCACTA TGCTCGATCT 841 TTCCTACAAC AACTTAAATG TGGTTGGTAA CGATTCCTTT GCTTGGCTTC CACAACTAGA 901 ATATTTCTTC CTAGAGTATA ATAATATACA GCATTTGTTT TCTCACTCTT TGCACGGGCT 961 TTTCAATGTG AGGTACCTGA ATTTGAAACG GTCTTTTACT AAACAAAGTA TTTCCCTTGC 1021 CTCACTCCCC AAGATTGATG ATTTTTCTTT TCAGTGGCTA AAATGTTTGG AGCACCTTAA 1081 CATGGAAGAT AATGATATTC CAGGCATAAA AAGCAATATG TTCACAGGAT TGATAAACCT 1141 GAAATACTTA AGTCTATCCA ACTCCTTTAC AAGTTTGCGA ACTTTGACAA ATGAAACATT 1201 TGTATCACTT GCTCATTCTC CCTTACACAT ATTCAACCTA ACCAAGAATA AAATCTCAAA 1261 AATAGAGAGT GATGCTTTCT CTTGGTTGGG CCACCTAGAA GTACTTGACC TGGGCCTTAA 1321 TGAAATTGGG CAAGAACTCA CAGGCCAGGA ATGGAGAGGT CTAGAAAATA TTTTCGAAAT 1381 CTATCTTTCC TACAACAAGT ACCTGCAGCT GACTAGGAAC TCCTTTGCCT TGGTCCCAAG 1441 CCTTCAACGA CTGATGCTCC GAAGGGTGGC CCTTAAAAAT GTGGATAGCT CTCCTTCACC 1501 ATTCCAGCCT CTTCGTAACT TGACCATTCT GGATCTAAGC AACAACAACA TAGCCAACAT 1561 AAATGATGAC ATGTTGGAGG GTCTTGAGAA ACTAGAAATT CTCGATTTGC AGCATAACAA 1621 CTTAGCACGG CTCTGGAAAC ACGCAAACCC TGGTGGTCCC ATTTATTTCC TAAAGGGTCT 1681 GTCTCACCTC CACATCCTTA ACTTGGAGTC CAACGGCTTT GACGAGATCC CAGTTGAGGT 1741 CTTCAAGGAT TTATTTGAAC TAAAGATCAT CGATTTAGGA TTGAATAATT TAAACACACT 1801 TCCAGCATCT GTCTTTAATA ATCAGGTGTC TCTAAAGTCA TTGAACCTTC AGAAGAATCT 1861 CATAACATCC GTTGAGAAGA AGGTTTTCGG GCCAGCTTTC AGGAACCTGA CTGAGTTAGA 1921 TATGCGCTTT AATCCCTTTG ATTGCACGTG TGAAAGTATT GCCTGGTTTG TTAATTGGAT 1981 TAACGAGACC CATACCAACA TCCCTGAGCT GTCAAGCCAC TACCTTTGCA ACACTCCACC 2041 TCACTATCAT GGGTTCCCAG TGAGACTTTT TGATACATCA TCTTGCAAAG ACAGTGCCCC 2101 CTTTGAACTC TTTTTCATGA TCAATACCAG TATCCTGTTG ATTTTTATCT TTATTGTACT 2161 TCTCATCCAC TTTGAGGGCT GGAGGATATC TTTTTATTGG AATGTTTCAG TACATCGAGT 2221 TCTTGGTTTC AAAGAAATAG ACAGACAGAC AGAACAGTTT GAATATGCAG CATATATAAT 2281 TCATGCCTAT AAAGATAAGG ATTGGGTCTG GGAACATTTC TCTTCAATGG AAAAGGAAGA 2341 CCAATCTCTC AAATTTTGTC TGGAAGAAAG GGACTTTGAG GCGGGTGTTT TTGAACTAGA 2401 AGCAATTGTT AACAGCATCA AAAGAAGCAG AAAAATTATT TTTGTTATAA CACACCATCT 2461 ATTAAAAGAC CCATTATGCA AAAGATTCAA GGTACATCAT GCAGTTCAAC AAGCTATTGA 2521 ACAAAATCTG GATTCCATTA TATTGGTTTT CCTTGAGGAG ATTCCAGATT ATAAACTGAA 2581 CCATGCACTC TGTTTGCGAA GAGGAATGTT TAAATCTCAC TGCATCTTGA ACTGGCCAGT 2641 TCAGAAAGAA CGGATAGGTG CCTTTCGTCA TAAATTGCAA GTAGCACTTG GATCCAAAAA 2701 CTCTGTA
Quality Control: ORF sequencing results is available upon request.
