Mouse TLR3 Gene cDNA Clone / ORF Clone

TLR3 cDNA Clone Datasheet

TLR3 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_126166.4
• cDNA Size: 2718
• Gene Synonym: AI957183
• Source: Mouse

TLR3 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus toll-like receptor 3 DNA.
• Vector: Vector pMD18-T Simple
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

TLR3 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TLR3 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

TLR3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAAGGGT GTTCCTCTTA TCTAATGTAC TCCTTTGGGG GACTTTTGTC CCTATGGATT
61       CTTCTGGTGT CTTCCACAAA CCAATGCACT GTGAGATACA ACGTAGCTGA CTGCAGCCAT
121      TTGAAGCTAA CACACATACC TGATGATCTT CCCTCTAACA TAACAGTGTT GAATCTTACT
181      CACAACCAAC TCAGAAGATT ACCACCTACC AACTTTACAA GATACAGCCA ACTTGCTATC
241      TTGGATGCAG GATTTAACTC CATTTCAAAA CTGGAGCCAG AACTGTGCCA AATACTCCCT
301      TTGTTGAAAG TATTGAACCT GCAACATAAT GAGCTCTCTC AGATTTCTGA TCAAACCTTT
361      GTCTTCTGCA CGAACCTGAC AGAACTCGAT CTAATGTCTA ACTCAATACA CAAAATTAAA
421      AGCAACCCTT TCAAAAACCA GAAGAATCTA ATCAAATTAG ATTTGTCTCA TAATGGTTTA
481      TCATCTACAA AGTTGGGAAC GGGGGTCCAA CTGGAGAACC TCCAAGAACT GCTCTTAGCA
541      AAAAATAAAA TCCTTGCGTT GCGAAGTGAA GAACTTGAGT TTCTTGGCAA TTCTTCTTTA
601      CGAAAGTTGG ACTTGTCATC AAATCCACTT AAAGAGTTCT CCCCGGGGTG TTTCCAGACA
661      ATTGGCAAGT TATTCGCCCT CCTCTTGAAC AACGCCCAAC TGAACCCCCA CCTCACAGAG
721      AAGCTTTGCT GGGAACTTTC AAACACAAGC ATCCAGAATC TCTCTCTGGC TAACAACCAG
781      CTGCTGGCCA CCAGCGAGAG CACTTTCTCT GGGCTGAAGT GGACAAATCT CACCCAGCTC
841      GATCTTTCCT ACAACAACCT CCATGATGTC GGCAACGGTT CCTTCTCCTA TCTCCCAAGC
901      CTGAGGTATC TGTCTCTGGA GTACAACAAT ATACAGCGTC TGTCCCCTCG CTCTTTTTAT
961      GGACTCTCCA ACCTGAGGTA CCTGAGTTTG AAGCGAGCAT TTACTAAGCA AAGTGTTTCA
1021     CTTGCTTCAC ATCCCAACAT TGACGATTTT TCCTTTCAAT GGTTAAAATA TTTGGAATAT
1081     CTCAACATGG ATGACAATAA TATTCCAAGT ACCAAAAGCA ATACCTTCAC GGGATTGGTG
1141     AGTCTGAAGT ACCTAAGTCT TTCCAAAACT TTCACAAGTT TGCAAACTTT AACAAATGAA
1201     ACATTTGTGT CACTTGCTCA TTCTCCCTTG CTCACTCTCA ACTTAACGAA AAATCACATC
1261     TCAAAAATAG CAAATGGTAC TTTCTCTTGG TTAGGCCAAC TCAGGATACT TGATCTCGGC
1321     CTTAATGAAA TTGAACAAAA ACTCAGCGGC CAGGAATGGA GAGGTCTGAG AAATATATTT
1381     GAGATCTACC TATCCTATAA CAAATACCTC CAACTGTCTA CCAGTTCCTT TGCATTGGTC
1441     CCCAGCCTTC AAAGACTGAT GCTCAGGAGG GTGGCCCTTA AAAATGTGGA TATCTCCCCT
1501     TCACCTTTCC GCCCTCTTCG TAACTTGACC ATTCTGGACT TAAGCAACAA CAACATAGCC
1561     AACATAAATG AGGACTTGCT GGAGGGTCTT GAGAATCTAG AAATCCTGGA TTTTCAGCAC
1621     AATAACTTAG CCAGGCTCTG GAAACGCGCA AACCCCGGTG GTCCCGTTAA TTTCCTGAAG
1681     GGGCTGTCTC ACCTCCACAT CTTGAATTTA GAGTCCAACG GCTTAGATGA AATCCCAGTC
1741     GGGGTTTTCA AGAACTTATT CGAACTAAAG AGCATCAATC TAGGACTGAA TAACTTAAAC
1801     AAACTTGAAC CATTCATTTT TGATGACCAG ACATCTCTAA GGTCACTGAA CCTCCAGAAG
1861     AACCTCATAA CATCTGTTGA GAAGGATGTT TTCGGGCCGC CTTTTCAAAA CCTGAACAGT
1921     TTAGATATGC GCTTCAATCC GTTCGACTGC ACGTGTGAAA GTATTTCCTG GTTTGTTAAC
1981     TGGATCAACC AGACCCACAC TAATATCTCT GAGCTGTCCA CTCACTACCT CTGTAACACT
2041     CCACATCATT ATTATGGCTT CCCCCTGAAG CTTTTCGATA CATCATCCTG TAAAGACAGC
2101     GCCCCCTTTG AACTCCTCTT CATAATCAGC ACCAGTATGC TCCTGGTTTT TATACTTGTG
2161     GTACTGCTCA TTCACATCGA GGGCTGGAGG ATCTCTTTTT ACTGGAATGT TTCAGTGCAT
2221     CGGATTCTTG GTTTCAAGGA AATAGACACA CAGGCTGAGC AGTTTGAATA TACAGCCTAC
2281     ATAATTCATG CCCATAAAGA CAGAGACTGG GTCTGGGAAC ATTTCTCCCC AATGGAAGAA
2341     CAAGACCAAT CTCTCAAATT TTGCCTAGAA GAAAGGGACT TTGAAGCAGG CGTCCTTGGA
2401     CTTGAAGCAA TTGTTAATAG CATCAAAAGA AGCCGAAAAA TCATTTTCGT TATCACACAC
2461     CATTTATTAA AAGACCCTCT GTGCAGAAGA TTCAAGGTAC ATCACGCAGT TCAGCAAGCT
2521     ATTGAGCAAA ATCTGGATTC AATTATACTG ATTTTTCTCC AGAATATTCC AGATTATAAA
2581     CTAAACCATG CACTCTGTTT GCGAAGAGGA ATGTTTAAAT CTCATTGCAT CTTGAACTGG
2641     CCAGTTCAGA AAGAACGGAT AAATGCCTTT CATCATAAAT TGCAAGTAGC ACTTGGATCT
2701     CGGAATTCAG CACATTAA

 

Quality Control:cDNA sequencing results is available upon request.