Mouse TLR3 / CD283 Protein (His Tag)

Mouse Toll-Like Receptor 3 ( TLR3 / CD283 ) Protein

TLR3 / CD283 Protein Product Information

• Synonym: AI957183, Tlr3
• Protein Construction: A DNA sequence encoding the soluble form of mouse TLR3 (NP_569054.2) extracellular domain(Cys 29 - Leu 705)was fused with a signal peptide at the N-terminus and a polyhistidine tag at the C-terminus
• Source: Mouse
• Expression Host: Baculovirus-Insect cells

TLR3 / CD283 Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Cys 29
• Molecular Mass: The secreted recombinant mouse TLR3 consists of 688 amino acids and has a calculated molecular mass of 78 kDa. The recombinant protein migrates as an approximately 75-80 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 50mM Tris, 100mM NaCl, 0.5mM PMSF, 10% glycerol, pH 8.5
• SDS-PAGE: TLR3 protein

TLR3 / CD283 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

TLR3 / CD283 Protein Description

Mouse Toll-Like Receptor 3, also known as CD283 and TLR3, is a member of the Toll-like receptor family. It contains 22 LRR (leucine-rich) repeats and 1 TIR domain. TLR3 is expressed at high level in placenta and pancreas. It is expressed most strongly in the brain, especially in astrocytes, glia, and neurons. TLR3 participates in the innate immune response to microbial agents. It is the major mediator of cellular response to viral infection, because it responds to double-stranded (ds) RNA, a common by-product of viral replication. Toll-like receptors (TLRs) play key roles in the response to pathogens and in mammals the host response to virus critically relies on TLR3 to detect viral-derived dsRNA. TLR3 acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Defects in TLR3 are the cause of TLR3-deficient herpes simplex encephalitis (HSE). HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. Genetic variation in TLR3 is associated with susceptibility to progression to geographic atrophy in age-related macular degeneration.

References

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6. Karpala, AJ. et al., 2008, Dev Comp Immunol  32 (4): 435-44.