TLR3 / CD283 Antibody (Antigen Affinity Purified)

Mouse Toll-Like Receptor 3 Antibody ( Antigen Affinity Purified )

Mouse TLR3 / CD283 Antibody Product Information

• Immunogen: Recombinant mouse TLR3 protein ( Catalog#50161-M08H )
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant mouse TLR3 ( rM TLR3 ; Catalog#50161-M08H ; NP_569054.2 ; Met 1 - Leu 705 ). TLR3 specific IgG was purified by mouse TLR3 affinity chromatography

Mouse TLR3 / CD283 Antibody Usage Guide

• Specificity: Mouse TLR3 / CD283
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse TLR3 in WB. Using a DAB detection system, the detection limit for mouse TLR3 is approximately 1 ng/lane under non-reducing conditions and 0.5 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Mouse TLR3. The detection limit for Mouse TLR3 is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Mouse TLR3 / CD283 Antibody Background

Mouse Toll-Like Receptor 3, also known as CD283 and TLR3, is a member of the Toll-like receptor family. It contains 22 LRR (leucine-rich) repeats and 1 TIR domain. TLR3 is expressed at high level in placenta and pancreas. It is expressed most strongly in the brain, especially in astrocytes, glia, and neurons. TLR3 participates in the innate immune response to microbial agents. It is the major mediator of cellular response to viral infection, because it responds to double-stranded (ds) RNA, a common by-product of viral replication. Toll-like receptors (TLRs) play key roles in the response to pathogens and in mammals the host response to virus critically relies on TLR3 to detect viral-derived dsRNA. TLR3 acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Defects in TLR3 are the cause of TLR3-deficient herpes simplex encephalitis (HSE). HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. Genetic variation in TLR3 is associated with susceptibility to progression to geographic atrophy in age-related macular degeneration

References

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