Mouse TLR2 Protein (His Tag) (Cytokine)

Toll-like receptor 2 Protein Datasheet

TLR2 Protein Product Information

• Synonym: TLR2
• Protein Construction: A DNA sequence encoding the mouse TLR2(Q9QUN7) (Met1-Gln587) was fused with a polyhistidine tag at the C-terminus.
• Source: Mouse
• Expression Host: Baculovirus-Insect cells

TLR2 Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gln 25
• Molecular Mass: The recombinant mouse TLR2 consists of 573 amino acids and has a calculated molecular mass of 64.8 kDa. The recombinant protein migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH7.4, 10%gly.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: TLR2 protein

TLR2 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

TLR2 Protein Description

TLR2, also known as CD282, belongs to the Toll-like receptor (TLR) family. Toll-like receptors (TLRs) are single transmembrane cell-surface receptors, which have a key role in the innate immune system. TLRs generally exist as homodimers and are found on immune cells such as macrophages, B lymphocytes and mast cells. Toll-like receptors are activated by molecules associated with biological threat and are highly specific towards evolutionary conserved entities on microbes, such as bacterial cell-surface lipopolysaccharides, flagella and unmethylated CpG islands. TLR2 is highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. It is also detected in lung and in fetal liver. Levels are low in other tissues. TLR2 cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. It also cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. TLR2 may also promote apoptosis in response to lipoproteins. CD282 recognizes mycoplasmal macrophage-activating lipopeptide-2kD, soluble tuberculosis factor, phenol-soluble modulin and B.burgdorferi outer surface protein A lipoprotein cooperatively with TLR6.

References

1. Do KN. et al., 2012, PLoS ONE. 7 (3): e32309.
2. Dziarski R. et al., 2001, J Endotoxin Res. 6( 5): 401-5.
3. Lorenz E. 2007, Curr Pharm Des. 12 (32): 4185-93.