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Rat TLR10 Gene cDNA Clone (full-length ORF Clone)

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TLR10cDNA Clone Product Information
Gene Bank Ref.ID:NM_001146035.1
cDNA Size:2435
cDNA Description:ORF Clone of Rattus norvegicus toll-like receptor 10 DNA.
Gene Synonym:Tlr10
Species:Rat
Vector:pGEM-T Vector
Restriction Site:
Tag Sequence:
Sequence Description:Same as Gene Bank Ref. ID sequence with the nucleotide 590 T deletion mutation and the point mutations499 T/C,1580 T/C,1637 A/G,2222 A/C,2285 G/A,resulting in the amino acid Phe substitution by Leu, Ile substitution by Met, Ile substitution by Thr,Glu substitution by Gly, Lys substitution by Thr, Arg substitution by His, and 381 C/T not causing the amino acid variation.
Shipping Carrier:Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.
Storage:The Whatman FTA elute card can be stored at room temperature for three months under dry condition.
pGEM-T vector information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
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Catalog:RG80402-G
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