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Rat TLR10 Gene cDNA Clone / ORF Clone
| Catalog | Vector (Price) | Quantity | In Stock | Operation |
| RG80402-G |
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YES |  |
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* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. |
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TLR10 cDNA Clone Datasheet
TLR10 cDNA Clone Product Information
| Gene Bank Ref.ID: | NM_001146035.1 |
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cDNA Size: |
2435 |
| Gene Synonym: |
Tlr10 |
| Source: | Rat |
TLR10 cDNA Clone Description
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cDNA Description: |
ORF Clone of Rattus norvegicus toll-like receptor 10 DNA. |
| Vector: | Cloning Vector: pGEM-T Vector
Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG |
| Sequence Description: | Same as Gene Bank Ref. ID sequence with the nucleotide 590 T deletion mutation and the point mutations499 T/C，1580 T/C，1637 A/G，2222 A/C，2285 G/A，resulting in the amino acid Phe substitution by Leu, Ile substitution by Met, Ile substitution by Thr，Glu substitution by Gly, Lys substitution by Thr, Arg substitution by His, and 381 C/T not causing the amino acid variation. |
TLR10 cDNA Clone Shipping
- 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
- It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
TLR10 cDNA Clone Usage Protocol
- Cut along the circle edge of the spot (arrow points to) to get the filter paper.
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to incubation at 95℃ for 15-30 min.
- At the end of the incubation pulse vortex, or gently tap the tube.
- Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.
TLR10 cDNA Clone Sequence and Quality Control
Sequence:
1 ATGAGACTCA TCAGCAGCAT CTATACCCTT TATAACATCA TTATGTCAAC AGAAGCCTGG 61 GCTCCAAGAC CCACAGATGA AAGGGAACTG ATCTCCAACT ACTCCAACAT GGCTTTAAGG 121 AAGGTTCCCC CAGATGTGAC CCCAGCCACA ACCATACTGG ATTTATCCTA CAACCTCCTC 181 TATCAATTCC AGAGCTCAGA TTTCCACTCC TTCTCCAAAC TGCGTTTTCT GATCCTGTGC 241 CATAACAGCA TCCAACAGCT GGACACCAAG ATCTTTGACC TTAACAAGGA GCTAAGCTAT 301 TTAGACTTGT CTTACAATAG ACTACAGTCC ATCACTTGGT CTTCCTTGGT AGATCTCAGA 361 CATTTAGATC TTTCTTTTAA TGACTTCGAT ACTCTGCCTG CCTGTGAGGA GATTGGCAAC 421 ATGCGACATC TGGAAACCTT AGGTTTGAGT GGGGCCAAAA TCCAAAGATC AGATCTCCAG 481 AAAATTGCTA ATTTGCAACT CAGCACTGTG TTCATAGGGC TGAATACTCT TTCTCACTAT 541 GAAGAAGGCA GCCTGCCTGT CCTAAACACG ACAGTGCTTC ACATAGTTTA CCAATGGCTG 601 CTAACTTCTG GGTGCTTCTG TGTGATGGAA TGAAGACTTC AAAGGTAGTC GAAATGTCAA 661 ACATAGGTGG CAAGAGCCAG TTTGTAAGTT ATGAGACTCC GAGAACTCTT TCTGTGGAGA 721 ATACCAAGGC GTCCACTCTT ATATTCCATA AAGTTGACCT GCTCTGGGAT GACCTTCTCC 781 ACGTTTTTCA GTTTGTGTGG CAGACTTCAG TGGAATGCTT CCAGATTCAG GACCTGACTT 841 TTGGAGGGAT GGCTAGTCGG GACCACAGTT CATTTGACTA CTCAAATACC GTAATGAAAG 901 CTGTAATCTT GGAGAGCATA CATTTCAGAA TCTTTTTCAT TCCCCAGAAT AAAATCTACT 961 TGTTTTTCAC CCAAATGGAT TTAGAAAACC TGACGATATC AGATGCACAA ATGCCACATC 1021 TTCTCTTCCC TAGTCACCCC CAAAGTTTCC AACATTTAAA TTTTGCCAAT AACATCTTAA 1081 CAGATGACCT GTTTAAAAAC CCCATCCAGA TGCCTTATCT GAAAACTCTC GTTTTGAGGG 1141 GCAACCAACT GGAGACTCTT TCTCTAGTGA GTCTCTTTGC CAACCACACG TCCTTGCTTC 1201 ACCTGGATCT GAGCCAGAAC CTATTGCAAC ATGGAAACGG GGAGAAATGC ATGTGGCCCG 1261 ACACCTTGAT CCTCTTGAAC TTGTCATCCA ATAAATTTGA TGGTTCTGTT TTCAGATGTT 1321 TGCCTAAAAG TATCCAGACA CTTGATCTGA ACAATAACAA AATTCGAGCA GTCCCTAAAG 1381 AGATTATATA CCTAAAATCT TTACGAGAGC TAAGTATTGC TTTTAATTTT CTGACTGACC 1441 TCCCTGGGTG TGATCACTTC AAACGGCTCT CTAGTCTGAA CATTGAAATG AATTTGATTC 1501 TCAGCCCATC TCTGGATTTT TTCCAGAGCT GCCAGGAGGT AAAGACTCTA AACGCAGGGC 1561 GAAATCCATT CCGGTGTACC TGTGAATTTA GAGACTTCAT TTGGCTTGAG AAACATTCCA 1621 AGGGCCTGAT GTCTGGATGG CCAGACTCCT ACGCCTGCGA ATACCCTTTG AGTCTAAAGG 1681 GGACTCCATT AAAAGATGTT TATCTTCCCG AATTAGCTTG CAACACAACT TTGCTGATTG 1741 TCACCATTGT GCTGGTTATC CTGGTTCTGG GTTTGGTTGT AGCATTCTGC ATTCTAAAAT 1801 TTGACATGCT TTGGTATCTC AGGGTGCTGG CCCAGTGCAT CCGGACACAG CATAGGGTTA 1861 GGAAGACAGT TCGAGAGGAG CGTAAGAGAA GTGTACAGTT CCATGCATTT ATCTCCTACG 1921 CTGCACATGA TTCTGTCTGG GTGAAGAATG AACTGATTCC CAATCTGGAG ACAGAAGATG 1981 GCTCTGTCTT GCTTTGTCTT CCCGAGAGGA ACTTTGACCC TGACAGGAGC ATTGCTGAAA 2041 ATATTATAAG CTGCACTGAG AAAAGCTATA AGTCCATCTT TGTGCTGTCC CCCAACTTTG 2101 TGCAGAATGG GTGGTGCCAT AATGAACTCT ATCTGGCCCA CCACAATCCT TTCCGTGACA 2161 ATTATGATTA CATCATTCCC ATCTTACTAG AACCCATTCC CTCCTACTGT GTTCCTACCA 2221 CGTTCCCTAA GCTGAAAGCT CTTTTGGAAA AGAAAGTATA CTTAGAGTGG CCCAAGGACA 2281 GGCACAAACA TGGGCTTTTT TGGGCAAACC TTCGAGCTGC CATTAACGTA AATTTATCAG 2341 ACACAAGGGG AAGGTGTGAA CTACAGACAT TCACAAAGCC TAACGCGGAT TGCCAAGGTT 2401 CTACCATCTC TCTGGTAAGA ACAGACAGCT TCTAA
Quality Control:cDNA sequencing results is available upon request.
