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Rat TLR10 Gene ORF cDNA clone in cloning vector

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Rat TLR10 cDNA Clone Product Information
NCBI RefSeq:NM_001146035.1
RefSeq ORF Size:2435
cDNA Description:ORF Clone of Rattus norvegicus toll-like receptor 10 DNA.
Gene Synonym:Tlr10
Species:Rat
Vector:pGEM-T Vector
Restriction Site:
Tag Sequence:
Sequence Description:Same as Gene Bank Ref. ID sequence with the nucleotide 590 T deletion mutation and the point mutations499 T/C,1580 T/C,1637 A/G,2222 A/C,2285 G/A,resulting in the amino acid Phe substitution by Leu, Ile substitution by Met, Ile substitution by Thr,Glu substitution by Gly, Lys substitution by Thr, Arg substitution by His, and 381 C/T not causing the amino acid variation.
Antibiotic in E.coli:Ampicilin
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
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