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TLK2/PKU-ALPHA Protein, Antibody, ELISA Kit, cDNA Clone

TLK2/PKU-ALPHA Related Areas

TLK2/PKU-ALPHA Related Pathways

TLK2/PKU-ALPHA Related Product

    TLK2/PKU-ALPHA Summary & Protein Information

    TLK2/PKU-ALPHA Background

    Gene Summary: The Tousled-like kinases, first described in Arabidopsis, are nuclear serine/threonine kinases that are potentially involved in the regulation of chromatin assembly.[supplied by OMIM, Apr 2004]
    General information above from NCBI
    Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
    Cofactor: Magnesium.
    Enzyme regulation: Cell cycle-regulated, with maximal activity in the S-phase. Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S- phase, probably by CHEK1, possibly at Ser-750. This inhibition is cell cycle checkpoint- and ATM-dependent.
    Subunit structure: Monomer and heterodimer with TLK1. Interacts with ASF1A and ASF1B. Association with 14-3-3 proteins such as YWHAZ regulates subcellular location. May also interact with FEZ1/LZTS1 and FEZ2.
    Subcellular location: Nucleus. Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton. Note=Colocalizes with the cytoplasmic intermediate filament system during the G1 phase of the cell cycle. Present in the perinuclear region at S phase and in the nucleus at late G2.
    Tissue specificity: Ubiquitous. Detected in placenta, fetal liver, kidney, pancreas, heart and skeletal muscle. Highly expressed in testis. Detected in spleen, thymus, colon, ovary, small intestine, prostate and peripheral blood leukocytes.
    Post-translational: Phosphorylated at Ser-750, probably by CHEK1.
    Sequence similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
    General information above from UniProt

    Serine / threonine-protein kinase tousled-like 2, also known as PKU-alpha, Tousled-like kinase 2 and TLK2, is a nucleus protein which belongs to the protein kinase superfamily and Ser/Thr protein kinase family. The tousled-like kinases are an evolutionarily conserved family of proteins implicated in DNA repair, DNA replication and mitosis in metazoans and plants. Their absence from the yeasts and other eukaryotic 'microbes' suggests a specific role for them in the development of multicellular organisms. Tousled-like kinase 2 / TLK2 is widely expressed. It is present in fetal placenta, liver, kidney, pancreas, heart and skeletal muscle. It is also found in adult cell lines. Tousled-like kinase 2 / TLK2 contains one protein kinase domain. Tousled-like kinase 2 / TLK2 is rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly.

    TLK2/PKU-ALPHA Alternative Name

    MGC44450,PKU-ALPHA,TLK2, [human]
    RP23-456M3.2,Tlk,4933403M19Rik,PKUalpha,Tlk2, [mouse]

    TLK2/PKU-ALPHA Related Studies

  • Sillje HHW. et al., 2001,Curr Biol. 11: 1068-73.
  • Groth A. et al., 2003, EMBO J. 22: 1676-87.
  • Beausoleil SA. et al., 2004, Proc Natl Acad Sci. 101: 12130-5.
  • Mayya V. et al., 2009, Sci Signal. 2: RA46.
  • Oppermann FS. et al., 2009, Mol Cell Proteomics. 8: 1751-64.
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