Mouse TIMP2 Gene cDNA Clone / ORF Clone

TIMP2 cDNA Clone Datasheet

TIMP2 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_011594.3
• cDNA Size: 663
• Gene Synonym: Timp-2; D11Bwg1104e; Timp2
• Source: Mouse

TIMP2 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus tissue inhibitor of metalloproteinase 2 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

TIMP2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TIMP2 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

TIMP2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGCGCCG CGGCCCGCAG CCTCCGGCTG GCGCTCGGCC TCCTGCTGCT AGCCACGCTG
61       CTGCGCCCGG CCGACGCCTG CAGCTGCTCC CCGGTGCACC CGCAACAGGC GTTTTGCAAT
121      GCAGACGTAG TGATCAGAGC CAAAGCAGTG AGCGAGAAGG AGGTGGATTC CGGGAATGAC
181      ATCTATGGCA ACCCCATCAA GAGGATTCAG TATGAGATCA AGCAGATAAA GATGTTCAAA
241      GGACCTGACA AAGACATCGA GTTTATCTAC ACGGCCCCCT CTTCAGCAGT GTGCGGGGTC
301      TCGCTGGACG TTGGAGGAAA GAAGGAGTAT CTAATTGCAG GAAAGGCAGA AGGAGATGGC
361      AAGATGCACA TTACCCTCTG TGACTTCATT GTGCCCTGGG ACACGCTTAG CATCACCCAG
421      AAGAAGAGCC TGAACCACAG GTACCAGATG GGCTGTGAGT GCAAGATCAC TCGCTGTCCC
481      ATGATCCCTT GCTACATCTC CTCCCCGGAT GAGTGCCTCT GGATGGACTG GGTCACAGAG
541      AAGAGCATCA ATGGGCACCA GGCCAAGTTC TTCGCCTGCA TCAAGAGAAG TGATGGTTCT
601      TGCGCGTGGT ACCGCGGGGC GGCACCCCCC AAGCAAGAGT TTCTTGACAT CGAGGACCCG
661      TAA

 

Quality Control:cDNA sequencing results is available upon request.