TIMP-1 / TIMP1 Antibody

TIMP-1 / TIMP1 Antibody

TIMP-1 / TIMP1 Antibody Product Information

• Immunogen: Recombinant human TIMP1 protein ( Catalog #10934-H08H )
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 004
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human TIMP1 ( rh TIMP1; Catalog#10934-H08H ; NP_003245.1 ; Met 1 - Ala 207 ).

TIMP-1 / TIMP1 Antibody Usage Guide

• Specificity: Human TIMP1
• No cross-reactivity in ELISA with
  

  Human TIMP2

• Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human TIMP1 in WB. Using a DAB detection system, the detection limit for human TIMP1 is approximately 1 ng/lane under non-reducing conditions and 10 ng/lane under reducing conditions
• Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human TIMP1. The detection limit for human TIMP1 is approximately 0.00975 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

TIMP-1 / TIMP1 Antibody Related Products & Topics

Related Areas:

Enzyme>>Protease & Regulator>>Metalloprotease & Regulator>>Tissue Inhibitor of Metalloproteinase>>TIMP-1/TIMP1

Cancer>>Angiogenesis>>Tissue Inhibitor of Metalloproteinase>>TIMP-1/TIMP1

Cancer>>Cancer Biomarkers>>TIMP-1/TIMP1

Proteins:

Molecule	Species	Description	Cat. No
TIMP-1/TIMP1	Human	TIMP-1/TIMP1 Protein, Recombinant	10934-H08H
TIMP-1/TIMP1	Human	TIMP1/TIMP1 Protein, Recombinant	10934-HNAH
TIMP-1/TIMP1	Mouse	TIMP-1/TIMP1 Protein, Recombinant	50342-MNAH

Antibodies:

Molecule	Application	Description	Cat. No
Human TIMP-1/TIMP1	ELISA	Mouse Monoclonal Antibody	10934-MM02
Human TIMP-1/TIMP1	WB, ELISA	Mouse Monoclonal Antibody	10934-MM03
Human TIMP-1/TIMP1	ELISA	Rabbit Polyclonal Antibody	10934-RP01
Human TIMP-1/TIMP1	WB	Rabbit Polyclonal Antibody(Antigen Affinity Purified)	10934-RP04

TIMP-1 / TIMP1 Antibody Background

Tissue inhibitors of metalloproteinases (TIMP) family are natural inhibitors of the matrix metalloproteinases (MMPs), the zinc enzymes involved in extracellular matrix maintenance and remodeling. The TIMP family encompasses four members (TIMP1-4), and the inducible form TIMP-1/TIMP1 is a 28 kDa glycoprotein expressed by a variety of cell types. It inhibits most MMPs except membrane-type MMP subfamily by forming non-covalent binary complex, but also interacts with the hemopexin-like (PEX) domain of pro MMP-9. TIMP-1/TIMP1 has been identified as a multifunctional molecule with divergent functions. It exhibits keratinocyte and fibroblast growth factor-like activity and acts as a cell survival factor, and additionally participates in tumor metastasis, angiogenesis, inflammatory responses, as well as CNS homeostasis. Thus, TIMP-1/TIMP1 overexpression may serve to help identify patients with particularly aggressive disease for adjuvant treatments.

References

1. Osthues, A. et al., 1992, FEBS. Lett. 296: 16-20.
2. Aljada, IS. et al., 2004, J. Clin. Oncol. 16: 3218-29.
3. Hornebeck, W., 2004, Pathol. Biol. 51: 569-73.
4. Gardner, J. et al., 2003, J. Neurosci. Res. 74: 801-6.