TIM4 / TIMD4 Antibody

TIM4 / TIMD4 Antibody Datasheet

TIM4 / TIMD4 Antibody Product Information

• Immunogen: Recombinant Mouse TIM4 / TIMD4 protein (Catalog#50388-M08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 102
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse TIM4 / TIMD4 (rM TIM4 / TIMD4; Catalog#50388-M08H; NP_848874.3; Met 1-Thr 279).

TIM4 / TIMD4 Antibody Usage Guide

• Specificity: Mouse TIM4 / TIMD4
• No cross-reactivity in ELISA with

  Human TIMD4

  Human KIM1 (aa 1-135)

• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse TIMD4. The detection limit for Mouse TIMD4 is approximately 0.0195 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

TIM4 / TIMD4 Antibody Background

T-cell immunoglobulin and mucin domain-containing protein 4, also known as T-cell membrane protein 4, TIMD4, and TIM4, is a single-pass type I membrane protein which belongs to theimmunoglobulin superfamily and TIM family. TIM4 contains oneIg-like V-type (immunoglobulin-like) domain. It is expressed on dendritic cells and macrophages. TIM4 plays an important role in the proliferation of T helper type 2 (Th2) cells. TIM4 is involved in regulating T-cell proliferation and lymphotoxin signaling. It is a ligand for HAVCR1 / TIMD1. The expression of TIM4 in fibroblasts enhanced their ability to engulf apoptotic cells. TIM4 and TIM1 are phosphatidylserine receptors for the engulfment of apoptotic cells, and may also be involved in intercellular signalling in which exosomes are involved. Modulation of TIM4 production in dendritic cells (DCs) represents a novel therapeutic approach for the treatment of peanut allergy.

References

1. Miyanishi, M.et al., 2007, Nature. 450 (7168): 435-9.
2. Liu,T. et al., 2007, Mol Immunol. 44 (14): 3580-7.
3. Cai, P. et al., 2008, J Huazhong Univ Sci Technolog Med Sci. 28 (4): 447-50.
4. Cai, PC. et al., 2009,Tissue Antigens. 74 (1): 11-6.