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> ELISA > ELISA Pair Set > TIM-3 / HAVCR2 / TIMD3 ELISA Pair Set, Human ELISA Pair Set TIM-3 / HAVCR2 / TIMD3 ELISA Pair Set, Human ELISA Pair Set 
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| SEK10390 |
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Human TIM-3 / HAVCR2 / TIMD3 ELISA Pair Set
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Order or Inquire for TIM-3 / HAVCR2 / TIMD3 ELISA Pair Set |
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High affinity Human TIM-3 / HAVCR2 / TIMD3 ELISA Pair Set |
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Detection limit - 31.25 pg/ml |
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Affordable price and 30%-80% cost saving for Bulk order |
- Materials
- Protocol
- Trouble Shooting
- Image & Flash
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No signal |
Incorrect or no Detection Antibody was added |
Add appropriate Detection Antibody and continue |
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Substrate solution was not added |
Add substrate solution and continue |
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Incorrect storage condition |
Check if the kit is stored at recommended condition and used before expiration date |
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Poor Standard Curve |
Standard was incompletely reconstituted or was inappropriately stored |
Aliquot reconstituted standard and store at -70 ℃ |
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Imprecise / inaccurate pipetting |
Check / calibrate pipettes |
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Incubations done at inappropriate temperature, timing or agitation |
Follow the general ELISA protocol |
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Background wells were contaminated |
Avoid cross contamination by using the sealer appropriately |
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Poor detection value |
The concentration of antigen in samples was too low |
Enriching samples to increase the concentration of antigen |
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Samples were ineffective |
Check if the samples are stored at cold environment. Detect samples in timely manner |
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High Background |
Insufficient washes |
Use multichannel pipettes without touching the reagents on the plate |
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Increase cycles of washes and soaking time between washes |
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TMB Substrate Solution was contaminated |
TMB Substrate Solution should be clear and colorless prior to addition to wells |
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Materials were contaminated |
Use clean plates, tubes and pipettes tips |
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Non-specificity |
Samples were contaminated |
Avoid cross contamination of samples |
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The concentration of samples was too high |
Try higher dilution rate of samples |
Problems 
Possible Sources 
Solutions 
Human TIM-3 / HAVCR2 / TIMD3 ELISA Pair Set Related Products & Topics
Related Areas:
Proteins:
Antibodies:
Background
Hepatitis A virus cellular receptor 2 ( HAVCR2 ), formerly known as T cell immunoglobulin and mucin domain-3 (TIM-3), is a transmembrane glycoprotein expressed on the surface of terminally differentiated Th1 cells but not on Th2 cells. TIM-3 thus serves as the surface molecule that distinguishes between the two effector populations derived from naive CD4(+) T-helper cells. TIM-3 is a member of the TIM family of immune regulating molecules with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. Th1 cells produce cytokines ( IFN-gamma, interleukin-2, TNF-alpha and lymphotoxin ) that are commonly associated with cell-mediated immune responses against intracellular pathogens, delayed-type hypersensitivity reactions, and induction of organ-specific autoimmune diseases. It has been identified that galectin-9 is the Tim-3 ligand, and the TIM-3-galectin-9 pathway may serve to inhibit Th1 cells during a normal immune response and may be crucial for the induction of peripheral tolerance. Dysregulation of the TIM-3-galectin-9 pathway could underlie chronic autoimmune disease states in human, such as multiple sclerosis.
