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> Gene Products > Human cDNA Clones > Human TIGIT/VSTM3 Gene cDNA Clone / ORF Clone Human TIGIT/VSTM3 Gene cDNA Clone / ORF Clone
| Catalog | Vector (Price) | Quantity | In Stock | Operation |
| HG10917-M |
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* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. |
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Manufactured By: Sino Biological Inc.
Gene Bank Ref.ID: NM_173799.2
cDNA Size: 735
Synonym: VSIG9;VSTM3; FLJ39873; DKFZp667A205
Source: Human. cDNA Description: ORF Clone of Homo sapiens T cell immunoreceptor with Ig and ITIM domains DNA.
Vector:Cloning Vector: pMD18-T Simple Vector.
Sequence Description: Identical with the Gene Bank Ref. ID sequence except for two point mutations:279T/C not causing the amino acid variation and 349G/A resulting in the amino acid 117Ala substitution by Thr. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
Quality Control: cDNA sequencing results is available upon request.
Gene Bank Ref.ID: NM_173799.2
cDNA Size: 735
Synonym: VSIG9;VSTM3; FLJ39873; DKFZp667A205
Source: Human. cDNA Description: ORF Clone of Homo sapiens T cell immunoreceptor with Ig and ITIM domains DNA.
Vector:
Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description: Identical with the Gene Bank Ref. ID sequence except for two point mutations:279T/C not causing the amino acid variation and 349G/A resulting in the amino acid 117Ala substitution by Thr. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
- Cut along the circle edge of the spot (arrow points to) to get the filter paper.
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to incubation at 95℃ for 15–30 min .
- At the end of the incubation pulse vortex, or gently tap the tube.
- Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .
Sequence:
1 ATGCGCTGGT GTCTCCTCCT GATCTGGGCC CAGGGGCTGA GGCAGGCTCC CCTCGCCTCA
61 GGAATGATGA CAGGCACAAT AGAAACAACG GGGAACATTT CTGCAGAGAA AGGTGGCTCT
121 ATCATCTTAC AATGTCACCT CTCCTCCACC ACGGCACAAG TGACCCAGGT CAACTGGGAG
181 CAGCAGGACC AGCTTCTGGC CATTTGTAAT GCTGACTTGG GGTGGCACAT CTCCCCATCC
241 TTCAAGGATC GAGTGGCCCC AGGTCCCGGC CTGGGCCTCA CCCTCCAGTC GCTGACCGTG
301 AACGATACAG GGGAGTACTT CTGCATCTAT CACACCTACC CTGATGGGAC GTACACTGGG
361 AGAATCTTCC TGGAGGTCCT AGAAAGCTCA GTGGCTGAGC ACGGTGCCAG GTTCCAGATT
421 CCATTGCTTG GAGCCATGGC CGCGACGCTG GTGGTCATCT GCACAGCAGT CATCGTGGTG
481 GTCGCGTTGA CTAGAAAGAA GAAAGCCCTC AGAATCCATT CTGTGGAAGG TGACCTCAGG
541 AGAAAATCAG CTGGACAGGA GGAATGGAGC CCCAGTGCTC CCTCACCCCC AGGAAGCTGT
601 GTCCAGGCAG AAGCTGCACC TGCTGGGCTC TGTGGAGAGC AGCGGGGAGA GGACTGTGCC
661 GAGCTGCATG ACTACTTCAA TGTCCTGAGT TACAGAAGCC TGGGTAACTG CAGCTTCTTC
721 ACAGAGACTG GTTAG
Quality Control: cDNA sequencing results is available upon request.
TIGIT (VSTM3) related areas, pathways, and other information