Mouse TIGIT Gene cDNA Clone / ORF Clone

TIGIT cDNA Clone Datasheet

TIGIT cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001146325.1
• cDNA Size: 726
• Gene Synonym: Vstm3; ENSMUSG00000071552; Tigit
• Source: Mouse

TIGIT cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus T cell immunoreceptor with Ig and ITIM domains DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

TIGIT cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TIGIT cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

TIGIT cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCATGGCT GGCTGCTCCT GGTCTGGGTC CAGGGGCTGA TACAGGCTGC CTTCCTCGCT
61       ACAGGAGCCA CAGCAGGCAC GATAGATACA AAGAGGAACA TCTCTGCAGA GGAAGGTGGC
121      TCTGTCATCT TACAGTGTCA CTTCTCCTCT GACACAGCTG AAGTGACCCA AGTCGACTGG
181      AAGCAGCAGG ACCAGCTTCT GGCCATTTAT AGTGTTGACC TGGGGTGGCA TGTCGCTTCA
241      GTCTTCAGTG ATCGGGTGGT CCCAGGCCCC AGCCTAGGCC TCACCTTCCA GTCTCTGACA
301      ATGAATGACA CGGGAGAGTA CTTCTGTACC TATCATACGT ATCCTGGTGG GATTTACAAG
361      GGGAGAATAT TCCTGAAGGT CCAAGAAAGC TCAGTGGCTC AGTTCCAGAC TGCCCCGCTT
421      GGAGGAACCA TGGCTGCTGT GCTGGGACTC ATTTGCTTAA TGGTCACAGG AGTGACTGTA
481      CTGGCTAGAA AGAAGTCTAT TAGAATGCAT TCTATAGAAA GTGGCCTTGG GAGAACAGAA
541      GCGGAGCCAC AGGAATGGAA CCTGAGGAGT CTCTCATCCC CTGGAAGCCC TGTCCAGACA
601      CAAACTGCCC CTGCTGGTCC CTGTGGAGAG CAGGCAGAAG ATGACTATGC TGACCCACAG
661      GAATACTTTA ATGTCCTGAG CTACAGAAGC CTAGAGAGCT TCATTGCTGT ATCGAAGACT
721      GGCTAA

 

Quality Control:cDNA sequencing results is available upon request.