|Catalog||Size (Price)||Quantity||In Stock||Operation||Other Information|
T cell immunoreceptor with Ig and ITIM domains Protein Datasheet
TIGIT/ VSTM3 Protein Price Inquiry ( Available Sizes )
TIGIT/ VSTM3 Protein Product Information
|Synonym :||TIGIT, Vstm3|
|Protein Construction:||A DNA sequence encoding the mouse TIGIT (PP86176) (Met1-Gly141 ) was expressed, fused with the Fc region of human IgG1 at the C-terminus.|
|Expression Host:||Human Cells|
TIGIT/ VSTM3 Protein QC Testing
|Purity:||> 90% as determined by SDS-PAGE||SDS-PAGE:
TIGIT/ VSTM3 protein
|Endotoxin:||< 1.0 EU per μg of the protein as determined by the LAL method|
|Stability:||Samples are stable for up to twelve months from date of receipt at -70℃|
|Predicted N terminal:||Gly 26|
The recombinant mouse TIGIT /Fc is a disulfide-linked homodimer. The reduced monomer comprises 357 amino acids and has a predicted molecular mass of 39.8 kDa. The apparent molecular mass of the protein is approximately 52 kDa in SDS-PAGE under reducing conditions due to glycosylation.
|Formulation:||Lyophilized from sterile PBS, pH7.4.
TIGIT/ VSTM3 Protein Usage Guide
|Storage:||Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|Reconstitution:||A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.|
TIGIT/ VSTM3 Protein Related Products & Topics
|Molecule||Species||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|TIGIT/VSTM3||Human||TIGIT/VSTM3 Protein, Recombinant||10917-H08H|
|TIGIT/VSTM3||Mouse||TIGIT / VSTM3 Protein, Recombinant||50939-M02H|
|TIGIT/VSTM3||Mouse||TIGIT / VSTM3 Protein, Recombinant||50939-M08H|
TIGIT/ VSTM3 Protein Description
TIGIT, also known as V-set and transmembrane domain-containing protein 3 (VSTM3) or V-set and immunoglobulin domain-containing protein 9 (VSIG9) is a new surface protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed on regulatory, memory, activated T cells and NK cells. It binds PVR with high affinity, and PVRL2 with lower affinity, but not PVRL3. Knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses, however, TIGIT inhibits NK cytotoxicity directly through its ITIM. TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. The binding of PVR to TIGIT on human dendritic cells enhanced the production of IL-10 and diminished the production of IL-12p40. In addition, TIGIT counter inhibits the NK-mediated killing of tumor cells and protects normal cells from NK-mediated cytotoxicity thus providing an "alternative self" mechanism for MHC class I inhibition.
- Yu X. et al., 2009, Nat Immunol. 10 (1): 48-57.
- Stanietsky N. et al., 2009, Proc Natl Acad Sci. 106 (42):17858-63.