Mouse THSD1 Gene cDNA Clone / ORF Clone

THSD1 cDNA Clone Datasheet

THSD1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_019576.1
• cDNA Size: 2556
• Gene Synonym: Tmtsp; AW121720; 4833423O18Rik; Thsd1
• Source: Mouse

THSD1 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus thrombospondin, type I , domain 1 DNA.
• Vector: Vector pMD18-T Simple
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except the point mutation 1635C/T not causing the amino acid variation.

THSD1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

THSD1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

THSD1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAACCCA TGTTGAAAGA CTTTTCAAAT CTCTTGCTGG TGGTGCTCTG TGACTATGTC
61       CTCGGAGAAG CCGAATACCT CCTCCTCCAA GAGCCAGTCC ATGTGGCACT GAGCGACAGA
121      ACGGTGTCAG TGGGTTTCCA CTACCTCAGT GACGTCAACG GGACACTGAG GAATGTGTCT
181      GTCATGCTGT GGGAGGCCAA CACCAATCGG ACTCTTACCA CTAAGTACCT CCTGACCAAC
241      CAGGCCCAAG GAACACTCCA GTTTGAATGT TTCTACTTCA AAGAGGCTGG TGACTACTGG
301      TTTGTAATGA TCCCGGAAGT GACAGACAAT GGCACGCAAG TTCCACTCTG GGAGAAAAGT
361      GCCTTTCTGA AGGTAGAATG GCCTGTCTTT CACATTGATT TAAATAGGAC AGCCAAGGCA
421      GCAGAAGGCA CCTTTCAAGT GGGTGTTTTT ACCACCCAAC CGCTCTGCCT GTTTCCCGTG
481      GACAAGCCAG ACATGCTGGT GGATGTTATT TTCACTGACC GTCTTCCGGA GGCAAGAGCA
541      AGTTTGGGAC AGCCGCTGGA GATCAGAGCC AGCAAAAGGA CAAAACTCAC TCAAGGTCAG
601      TGGGTCGAGT TTGGCTGTGC ACCGGTAGGG GTGGAAGCCT ACGTTACAGT CATGCTGAGG
661      CTGTTGGGTC AAGACTCAGT CATTGCTTCT ACGGGACCTA TTGACCTGGC TCAAAAATTC
721      GGATACAAAT TGATGATGGC ACCGGAAGTC ACGTGTGAGT CTGTGCTGGA GGTGATGGTA
781      CTGCCACCTC CTTGTGTCTT CGTCCAAGGA GTCCTGGCTG TTTACAAAGA AGCCCCCAAA
841      CGCCCGGAGG AGAGGACTTT CCAGGTGGCT GAAAACAGAC TGCCCCTGGG AGAGAGGAGA
901      ACGGTGTTCA ACTGCACTTT ATTTGATGTA GGGAAGAACA AATACTGTTT TAACTTTGGA
961      ATTGTGAAGA AAGGCCATTT TTCTGCAAAG GAATGCATGC TAATTCAGAG AAATATAGAA
1021     ACTTGGGGAC CATGGCAGCC GTGGAGCCCG TGTAGCACCA CGTGCGGGGA TGCTGTCCGA
1081     GAGCGTCGCC GCCTGTGTGT CACTTCTTTC CCCTCCAGAC CCAGCTGCTC TGGAATGTCC
1141     TCAGAGACCT CTCCATGCTC CCTGGAGGAG TGTGCTGTTT TCCGGCCACC AGGCCCATCG
1201     CCTGTTTCAC CCCAGGACCC TGTGAAGTCC AACAACGTGG TGACCGTCAC AGGGATCTCC
1261     CTGTGCCTGT TCATCATCTT TGCCACGGTG CTCATCACTC TCTGGAGGAG GTTTGGCCGA
1321     GCCCCCAAAT GCAGCACGCC CGTTCGCCAC AACTCCATCC ATTCCCCTGG CTTCCGGAAG
1381     AACTCCGATG AAGAGAACAT CTGCGAGCTG AGTGAGCCTC GCGGAAGCTT CTCGGATGCC
1441     GGTGACGGAC CTAGGGGAAG CCCAGGGGAC ACGGGCATCC CATTGACTTA CAGGTGCAGT
1501     GCATCAGCGC CTCCTGAGGA TGAGGCCTCG GGCAGTGAGA GCTTCCAGTC CAACGCTCAG
1561     AAGATCATCC CGCCCTTGTT TAGCTACCGC CTTGCCCAGC AGCAGCTGAA GGAGATGAAG
1621     AAGAAAGGGC TGACTGAGAC CACCAAAGTG TACCACGTAT CTCAAAGCCC CCTGACAGAC
1681     ACTGTAGTGG ATGCCACGGC CAGCCCTCCC TTAGACCTGG AATGCCCCGA AGAGGCTGCA
1741     GCAAGCAAGT TCCGAATCAA ATCTCCATTT CTGGACCAGC CTGGGGCAGG TACCGGGGAA
1801     AGGCCTCCCT CCAGGCTGGA TGGCATCGTG CCTCCTCCTG GCTGTGCGGT CAGTCCCAGC
1861     CAGACCCTGA TCCGAAAGTC ACAGATAAGG TCGACCGGTG GCAGAGATGG CTCATCGGAG
1921     AGGTGCCACT CCAGAAGTTC CCTCTTCAGG AGGACTGCTA GTTTTCATGA AACCAAGCAG
1981     TCTCGCCCTT TCCGGGAGAG GAGTTTGTCA GCCCTGACTC CCCGCCAGGT CCCCGCCTAC
2041     AGTTCCAGGA TGCGGACCTG GGACCAGATG GAGGATAGAT GTCGGCCTCC CAGTCGAAGT
2101     ACCCACCTGC TTCCAGAGAG ACCAGAGCAC TTCCAAGGGG CAGGTCGGAC CAGCAGTCCT
2161     TTGGGTCCAC TCTCCAAATC CTACACTGTG GGGCATCCCA GGAGGAAACC GGACCCAGGG
2221     GATCGTCAGG CCGGATTGGT GGCAGGAGCT GAGAAAATGG AGCCTCACCG AGCTCACAGG
2281     GGACCGTCCC CCAGTCACAG GAGTGCCTCA AGGAAGCAGT CTTCCCCCAT TTTCCTCAAA
2341     GATAGCTACC AGAAAGTCAG TCAGCTTAGC CCTTCTCACT TCAGAAAAGA TAAATGCCAG
2401     AGCTTCCCCA TCCACCCCGA GTTCGCCTTC TATGACAATA CCTCTTTCCG CCTCACCGAG
2461     GCTGAGCAGA GAATGCTGGA CCTCCCAGGA TACTTCGGCT CCAACGAAGA GGACGAAACC
2521     ACAAGTACAC TCAGTGTGGA GAAGTTAGTG ATCTAG

 

Quality Control:cDNA sequencing results is available upon request.