Mouse THSD1 / TMTSP Protein (His Tag)

Mouse Thrombospondin Type-1 Domain-Containing Protein 1

THSD1 / TMTSP Protein Product Information

• Synonym: Thsd1, 4833423O18Rik, AW121720, Tmtsp
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse THSD1 ( NP_062522.1 ) ( Met 1 - Asn 412 ) was expressed, with a C-terminal polyhistidine tag
• Source: Mouse
• Expression Host: Human Cells

THSD1 / TMTSP Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu 25
• Molecular Mass: The secreted recombinant mouse THSD1 consists of 399 amino acids and has a calculated molecular mass of 45 kDa. As a result of glycosylation, the apparent molecular mass of rm THSD1 is approximately 60-70 kDa in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: THSD1 protein

THSD1 / TMTSP Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

THSD1 / TMTSP Protein Description

Mouse thrombospondin type-1 domain-containing protein 1, also known as transmembrane molecule with thrombospondin module, THSD1 and TMTSP, is a single-pass type I  membrane protein which contains one TSP type-1 domain. Isoform 3 of THSD1 is a secreted protein. THSD1 is a good novel candidate TSG mapping to 13q14. Quantitative reverse transcription-PCR detected down-regulation of THSD1 expression in 100% of ESCC and other cancer cell lines. Mechanisms for THSD1 silencing in ESCC involved loss of heterozygosity and promoter hypermethylation, as analyzed by methylation-specific PCR and clonal bisulfite sequencing. Transfection of wild-type THSD1 into SLMT-1 resulted in significant reduction of colony-forming ability, hence providing functional evidence for its growth-suppressive activity.

References

1. Kis, E. et al., 2006, Int J Radiat Oncol Biol Phys 66 (5): 1506-14.
2. Ko,J.M. et al., 2008, Mol Cancer Res 6 (4): 592-603.