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THOP1 Antibody, Rabbit PAb, Antigen Affinity Purified
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THOP1 Antibody, Rabbit PAb, Antigen Affinity Purified PDF Download

Catalog   Size (Price) Quantity In Stock Operation
50828-RP02  

YES   
 

Primary antibody | Secondary antibody | Tag antibody | Isotype control antibody | Loading control antibody | Antibody Purification

THOP1 Antibody (Antigen Affinity Purified) Datasheet

  Order or Inquire for THOP1 Antibody product Quality antibodies Antibody production services
  Detection limit is 0.00975 ng/well in ELISA
 

THOP1 Antibody Product Information

Immunogen :

Recombinant Mouse THOP1 protein (Catalog#50828-M07B)

Antibody Type : Rabbit Polyclonal Antibody ( Antibody Purification Platform )
Ig Type :

Rabbit IgG

Formulation : 0.2 μm filtered solution in PBS, 5% trehalose may be added in some batches. Please read the hardcopy of COA or contact our customer service to confirm the formulation.
Preparation :

Produced in rabbits immunized with purified, recombinant Mouse THOP1 (rM THOP1; Catalog#50828-M07B; NP_073144.3; Lys 2-Cys687). THOP1 specific IgG was purified by Mouse THOP1 affinity chromatography.

THOP1 Antibody Usage Guide

Specificity :

Mouse THOP1

Western blot :

This antibody can be used at 5-10 μg/mL with the appropriate secondary reagents to detect Mouse THOP1 in WB.

THOP1 Western blot
  A B C
Sample
(whole cell lysate)
HCT116 HELA K562
Sample Volume
(cells/lane)
5×105 2.5×105 5×105
Gel 13% SDS-PAGE reducing gel
Recommended Concentration 5-10 μg/ml
Secondary Antibody Dylight 800-Labeled Antibody To Rabbit IgG (H+L), at 1:5000 dilution.
Direct ELISA : This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse THOP1. The detection limit for Mouse THOP1 is approximately 0.00975 ng/well.
Storage : This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

THOP1 Antibody Related Products & Topics

Related Areas:

Enzyme>>Protease & Regulator>>Metalloprotease & Regulator>>THOP1

Proteins:

Molecule Species Description //For Detailed Info. and Price------CLICK! Cat. No
THOP1 Mouse THOP1 Protein, Recombinant 50828-M07B

Antibodies:

Molecule Application Description //For Detailed Info. and Price------CLICK! Cat. No
Mouse THOP1 WB, ELISA THOP1 Antibody 50828-RP02

THOP1 Antibody Background

Protein Description Thimet oligopeptidase 1 (THOP1), also known as Thimet oligopeptidase, EC 3.4.24.15, or EP24.15. Human endopeptidase (THOP1) is localized on chromosome 19, which againsts toxic effects of Abeta in the early stages of Alzheimer disease (AD) pathology, and suggest that the observed increase in THOP1 expression might be part of a compensatory defense mechanism of the brain against an increased Abeta load. THOP1 was originally described as a neuropeptide-metabolizing enzyme, highly expressed in the brain, kidneys and neuroendocrine tissue. EP24.15 lacks a typical signal peptide sequence for entry into the secretory pathway and is secreted by cells via an unconventional and unknown mechanism. THOP1/EP24.15 actions are increased in androgen-dependent prostate cancer compared to androgen-independent; augmented by androgen treatment, and inhibited by clinical GnRH analogs.

References

  1. Meckelein B. et al., 1996, Genomics. 31 (2): 246-9.
  2. Torres MP. et al., 1998, Genomics. 53 (2): 239-40.
  3. Pollio G. et al., 2008, Neurobiol Dis. 31 (1): 145-58.
  4. Russo LC. et al., 2009, FEBS J. 276 (16): 4358-71.
  5. Swanson TA. et al., 2004, Protein Pept Lett. 11 (5): 471-8.
 
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"