THOP1 Antibody (Antigen Affinity Purified)

THOP1 Antibody (Antigen Affinity Purified) Datasheet

THOP1 Antibody Product Information

• Immunogen: Recombinant Mouse THOP1 protein (Catalog#50828-M07B)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse THOP1 (rM THOP1; Catalog#50828-M07B; NP_073144.3; Lys 2-Cys687). THOP1 specific IgG was purified by Mouse THOP1 affinity chromatography.

THOP1 Antibody Usage Guide

• Specificity: Mouse THOP1
• Western blot: This antibody can be used at 5-10 μg/mL with the appropriate secondary reagents to detect Mouse THOP1 in WB.

  A B C Sample (whole cell lysate) HCT116 HELA K562 Sample Volume (cells/lane) 5×105 2.5×105 5×105 Gel 13% SDS-PAGE reducing gel Recommended Concentration 5-10 μg/ml Secondary Antibody Dylight 800-Labeled Antibody To Rabbit IgG (H+L), at 1:5000 dilution.

• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse THOP1. The detection limit for Mouse THOP1 is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

THOP1 Antibody Background

Protein Description Thimet oligopeptidase 1 (THOP1), also known as Thimet oligopeptidase, EC 3.4.24.15, or EP24.15. Human endopeptidase (THOP1) is localized on chromosome 19, which againsts toxic effects of Abeta in the early stages of Alzheimer disease (AD) pathology, and suggest that the observed increase in THOP1 expression might be part of a compensatory defense mechanism of the brain against an increased Abeta load. THOP1 was originally described as a neuropeptide-metabolizing enzyme, highly expressed in the brain, kidneys and neuroendocrine tissue. EP24.15 lacks a typical signal peptide sequence for entry into the secretory pathway and is secreted by cells via an unconventional and unknown mechanism. THOP1/EP24.15 actions are increased in androgen-dependent prostate cancer compared to androgen-independent; augmented by androgen treatment, and inhibited by clinical GnRH analogs.

References

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3. Pollio G. et al., 2008, Neurobiol Dis. 31 (1): 145-58.
4. Russo LC. et al., 2009, FEBS J. 276 (16): 4358-71.
5. Swanson TA. et al., 2004, Protein Pept Lett. 11 (5): 471-8.