Anti-Thimet Oligopeptidase Antibody

Cat: 50828-RP02
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Anti-Thimet Oligopeptidase Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-Thimet Oligopeptidase Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Mouse
Specificity
Mouse Thimet Oligopeptidase
Immunogen
Recombinant Mouse THOP1 protein (Catalog#50828-M07B)
Preparation
Produced in rabbits immunized with purified, recombinant Mouse THOP1 (rM THOP1; Catalog#50828-M07B; NP_073144.3; Lys 2-Cys687). THOP1 specific IgG was purified by Mouse THOP1 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Thimet Oligopeptidase Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 4-6 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Thimet Oligopeptidase Antibody (Rabbit Polyclonal antibody) Images
Mouse THOP1 Immunoprecipitation(IP) 8257

Mouse THOP1 was immunoprecipitated using:

Lane A:0.5 mg K562 Whole Cell Lysate

2 µL anti-Mouse THOP1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Mouse THOP1 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 80 kDa

Observed band size: 80 kDa

Mouse THOP1 Western blot (WB) 13712

Anti-THOP1 rabbit polyclonal antibody at 1:500 dilution

Lane A: HCT116 Whole Cell Lysate

Lane B: HELA Whole Cell Lysate

Lane C: K562 Whole Cell Lysate

Lane D: 293T Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:79 kDa

Observed band size:72 kDa

Anti-Thimet Oligopeptidase Antibody Alternative Names
Anti-AI131655 Antibody;Anti-AI327041 Antibody;Anti-EP24.15 Antibody
Thimet Oligopeptidase Background Information

THOP1, also known as Thimet oligopeptidase 1, Thimet oligopeptidase, EC 3.4.24.15, or EP24.15, is a zinc(II) endopeptidase implicated in the processing of numerous physiological peptides. As an intracellular enzyme, highly expressed in the brain, kidneys and neuroendocrine tissue, THOP1 has been proposed to metabolize peptides within cells, thereby affecting antigen presentation and G protein-coupled receptor signal transduction. Its substrates is gonadotrophin-releasing hormone (GnRH), an important hypothalamic hormone that regulates the synthesis and release of oestradiol and facilitates female sexual behaviour. THOP1 against toxic effects of Abeta in the early stages of Alzheimer disease (AD) pathology, and suggest that the observed increase in THOP1 expression might be part of a compensatory defense mechanism of the brain against an increased Abeta load.

Full Name
thimet oligopeptidase 1
References
  • Cyr NE, et al. (2010) Nuclear Thimet oligopeptidase is coexpressed with oestrogen receptor alpha in hypothalamic cells and regulated by oestradiol in female mice. J Neuroendocrinol. 22(8): 936-43.
  • Berti DA, et al. (2009) Analysis of intracellular substrates and products of thimet oligopeptidase in human embryonic kidney 293 cells. J Biol Chem. 284(21): 14105-16.
  • Russo LC, et al. (2009) Interaction with calmodulin is important for the secretion of thimet oligopeptidase following stimulation. FEBS J. 276(16): 4358-71.
  • Pollio G, et al. (2008) Increased expression of the oligopeptidase THOP1 is a neuroprotective response to Abeta toxicity. Neurobiol Dis. 31(1): 145-58.
  • Bruce LA, et al. (2008) Hydrogen bond residue positioning in the 599-611 loop of thimet oligopeptidase is required for substrate selection. FEBS J. 275(22): 5607-17.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Thimet Oligopeptidase Antibody Rabbit Polyclonal ELISA 50828-RP01
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