- EGFR Signaling Pathway
- TGF-beta Signaling
- Canonical Wnt Signaling
- non-Canonical Wnt Signaling
- Notch Signaling
- p53 Pathway
- NF-kB Pathway
- Cytokine Signaling
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THOP1 Antibody (Antigen Affinity Purified) Datasheet
|Order or Inquire for THOP1 Antibody product||Quality antibodies||Antibody production services|
|Detection limit is 0.00975 ng/well in ELISA|
THOP1 Antibody Product Information
Recombinant Mouse THOP1 protein (Catalog#50828-M07B)
|Antibody Type :||Rabbit Polyclonal Antibody ( Antibody Purification Platform )|
|Ig Type :||
|Formulation :||0.2 μm filtered solution in PBS, 5% trehalose may be added in some batches. Please read the hardcopy of COA or contact our customer service to confirm the formulation.|
Produced in rabbits immunized with purified, recombinant Mouse THOP1 (rM THOP1; Catalog#50828-M07B; NP_073144.3; Lys 2-Cys687). THOP1 specific IgG was purified by Mouse THOP1 affinity chromatography.
THOP1 Antibody Usage Guide
|Western blot :||
This antibody can be used at 5-10 μg/mL with the appropriate secondary reagents to detect Mouse THOP1 in WB.
|Direct ELISA :||This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse THOP1. The detection limit for Mouse THOP1 is approximately 0.00975 ng/well.|
|Storage :||This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
THOP1 Antibody Related Products & Topics
|Molecule||Species||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|THOP1||Mouse||THOP1 Protein, Recombinant||50828-M07B|
|Molecule||Application||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|Mouse THOP1||WB, ELISA||THOP1 Antibody||50828-RP02|
THOP1 Antibody Background
Protein Description Thimet oligopeptidase 1 (THOP1), also known as Thimet oligopeptidase, EC 220.127.116.11, or EP24.15. Human endopeptidase (THOP1) is localized on chromosome 19, which againsts toxic effects of Abeta in the early stages of Alzheimer disease (AD) pathology, and suggest that the observed increase in THOP1 expression might be part of a compensatory defense mechanism of the brain against an increased Abeta load. THOP1 was originally described as a neuropeptide-metabolizing enzyme, highly expressed in the brain, kidneys and neuroendocrine tissue. EP24.15 lacks a typical signal peptide sequence for entry into the secretory pathway and is secreted by cells via an unconventional and unknown mechanism. THOP1/EP24.15 actions are increased in androgen-dependent prostate cancer compared to androgen-independent; augmented by androgen treatment, and inhibited by clinical GnRH analogs.
- Meckelein B. et al., 1996, Genomics. 31 (2): 246-9.
- Torres MP. et al., 1998, Genomics. 53 (2): 239-40.
- Pollio G. et al., 2008, Neurobiol Dis. 31 (1): 145-58.
- Russo LC. et al., 2009, FEBS J. 276 (16): 4358-71.
- Swanson TA. et al., 2004, Protein Pept Lett. 11 (5): 471-8.