Rat THBD Gene cDNA Clone / ORF Clone

THBD cDNA Clone Datasheet

THBD cDNA Clone Product Information

• Gene Bank Ref.ID: NM_031771.2
• cDNA Size: 1734
• Gene Synonym: Thbd
• Source: Rat

THBD cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus thrombomodulin DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 1650 A/G not causing the amino acid variation.

THBD cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

THBD cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O Briefly centrifuge the tube for 10 seconds.
3. Incubate the tube at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

THBD cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTTGGGG TTTTCCTTCT GGGTGTGCTG GCTCCAGCTG GCCTGGGGAT CTCTGCACTA
61       GCCAAGCTGC AGCCCAAAGG CAGTCAATGC GTGGGGAATG AGTGCTTCGC GCTTTTCCAG
121      GACCCCGTGA CCTTCCTCGA TGCCAGCCAG GCCTGCCAGC GCCTGCAGGG ACATTTGATG
181      ACAGTGCGCT CCTCGGTGGC TGCGGATGTC ATCTCCCTTC TGGTGAGCGA CAGTAGTATG
241      GACTCACGGC CCTGGATCGG TTTACAGCTC CCTCAGGGCT GTGGTGACCC GGTGCATCTC
301      GGGCCCCTGC GCGGCTTCCA GTGGGTTACT GGGGATAACC ACACCAGTTA CAGCAGGTGG
361      GCGCGGCCCA ACGACCAGTC GCCTCCACTT TGCGGTCCGC TGTGCGTCAC GGTCTCAACA
421      GCAACAGAAG CTGCACCAGG CGAGCCGGCC TGGGAAGAGA AGCCGTGCGA GAACGAGACC
481      AAGGGTTTTC TCTGCGAGTT TTACTTCGCA GCTTTCTGCA GGCCTTTACG GGTGAATACC
541      CGAGATCCTG AGGGTGCCCA CATCTCTAGC ACCTACAATA CCCCATTGGG GGTCAGCGGC
601      GCGGATTTTC AGACGCTGCC GATAGGCAGT TCTGCTACCG TGGCGCCCTT TGGCTTGGAG
661      CTGGTGTGCA GGGCCCTGCC CGGAACTTCA GAGGGACACT GGACTCGGGA AGTGACAGGA
721      GCTTGGAACT GCAGCGTGGA GAATGGTGGC TGCGAGTACA TGTGCAACAG GAGCGCTAAC
781      GGACCCAGAT GCGTCTGCCC CAGCGGCGGG GACCTGCAGG CTGATGGCCG TTCGTGCGCA
841      AAACCTGTGG CTCAATTGTG CAACGAACTC TGCCAGCATT TTTGCGTCAA CAACTCTGAT
901      GTGCCAGGCT CTTATTCCTG TATGTGCGAA ACAGGCTACC AGTTGGCGGC AGACGGACAT
961      CGGTGTGAGG ACGTGGATGA CTGTAAGCAG GGGCCCAATC CATGCCCTCA GCTCTGTTCT
1021     AACACCGAGG GCGGCTTCGA ATGCCGCTGC TATGATGGCT ATGAGTTGGT GGACGGAGAG
1081     TGCGTGGAGC AACTGGATCC GTGCTTCAGA TCTAAATGCG AGTATCAGTG CCAGCCAGTA
1141     AACTCCACGC ACTACAATTG CATCTGTGCT GAGGGCTTCG CACCTAAGCT GGATGATCCC
1201     GACAGGTGCG AAATGTTCTG CAATGAAACT TCGTGCCCAG CAGACTGTGA CCCCAACTCC
1261     CCAAGCTTTT GTCAATGCCC TGAGGGCTTC ATCCTGGACG AGGGTTCCAT ATGCACAGAC
1321     ATTGATGAGT GCAGTCAAGG CGAATGCCTC ACCAATGAAT GTCGAAACCT TCCTGGCTCC
1381     TATGAGTGCA TCTGCGGACC TGACACAGCC CTTGCTGGTC AGATTAGCAA GGACTGTGAC
1441     CCCATCCCTG TTCTGGAGGA CTCAGAGGAT GGTGGCTCTG GGGAGCACCC ATCAAGCAAT
1501     CCGACGGTAG TCTCTTCGAC AGTTCCCCCT TCTGCAAGAC CAATGCACTC TGGTGTGCTC
1561     ATTGGGATCT CCATTGCCAG CCTGTCCCTG GTGGTGGCGC TTTTGGCGCT TCTTTGTCAC
1621     CTGCGCAAGA AGCAGGGCAC TGCTCGCGCG GAGCTGGAGT ACAAGTGTAC CTCTTCAGCC
1681     AAGGAGGTAG TACTGCAGCA CGTGAGGACT GATCGGACGC TGCAGAAGTT CTGA

 

Quality Control:cDNA sequencing results is available upon request.