Anti-Tyrosine Hydroxylase Antibody

Cat: 50997-RP02
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Anti-Tyrosine Hydroxylase Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-Tyrosine Hydroxylase Antibody
Validated applications
ELISA,IHC-P
Species reactivity
Reacts with: Mouse
Specificity
Mouse Tyrosine Hydroxylase
Immunogen
Recombinant Mouse TH / Tyrosine Hydroxylase protein (Catalog#50997-M07B)
Preparation
Produced in rabbits immunized with purified, recombinant Mouse TH / Tyrosine Hydroxylase (rM TH / Tyrosine Hydroxylase; Catalog#50997-M07B; P24529; Pro 2-Ser 498). TH / Tyrosine Hydroxylase specific IgG was purified by Mouse TH / Tyrosine Hydroxylase affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Tyrosine Hydroxylase Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:5000-1:10000  
IHC-P 1:500-1:2000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Tyrosine Hydroxylase Antibody (Rabbit Polyclonal antibody) Images
Mouse TH / Tyrosine Hydroxylase Immunohistochemistry(IHC) 3171
Immunochemical staining of mouse TH in mouse brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Tyrosine Hydroxylase Background Information

Tyrosine hydroxylase (TH) is a rate-limiting enzyme in catecholamine synthesis. Tyrosine hydroxylase activity is modulated by protein-protein interactions with enzymes in the same pathway or the tetrahydrobiopterin pathway, structural proteins considered to be chaperones that mediate the neuron's oxidative state. It is phosphorylated at serine (Ser) residues Ser8, Ser19, Ser31 and Ser40 in vitro. The phosphorylation of tyrosine hydroxylase at Ser19 or Ser8 has no direct effect on tyrosine hydroxylase activity. As tyrosine hydroxylase (TH) catalyses the formation of L-DOPA, the rate-limiting step in the biosynthesis of DA, the Parkinson's disease (PD) can be considered as a TH-deficiency syndrome of the striatum. A direct pathogenetic role of TH has also been suggested, as the enzyme is a source of reactive oxygen species (ROS) in vitro and a target for radical-mediated oxidative injury. Recently, it has been demonstrated that L-DOPA is effectively oxidized by mammalian Tyrosine hydroxylase in vitro, possibly contributing to the cytotoxic effects of DOPA.

Full Name
tyrosine hydroxylase
References
  • Daubner SC, et al. (2011) Tyrosine hydroxylase and regulation of dopamine synthesis. Arch Biochem Biophys. 508(1): 1-12.
  • Dunkley PR, et al. (2004) Tyrosine hydroxylase phosphorylation: regulation and consequences. J Neurochem. 91(5): 1025-43.
  • Haavik J, et al. (1998) Tyrosine hydroxylase and Parkinson's disease. Mol Neurobiol. 16(3): 285-309.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Tyrosine Hydroxylase Antibody Rabbit Polyclonal ELISA 50997-RP01
    Anti-Tyrosine Hydroxylase Antibody Rabbit Monoclonal WB,ELISA 50997-R001
    Anti-Tyrosine Hydroxylase Antibody Rabbit Polyclonal WB,ICC/IF,IF,IP 100698-T38
    Anti-Tyrosine Hydroxylase Antibody Rabbit Polyclonal ELISA 10684-T16
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