Cynomolgus monkey TGFBR2 Gene cDNA Clone / ORF Clone (Cytokine)

TGFBR2 cDNA Clone Datasheet

TGFBR2 cDNA Clone Product Information

• cDNA Size: 1704
• Gene Synonym: TGFBR2
• Source: Cynomolgus monkey

TGFBR2 cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) transforming growth factor, beta receptor II (70/80kDa) DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 639 C/T，729 T/C，1128 G/C，1251 G/A and 1452 T/C not causing the amino acid variation.
  Please check the sequence information before order.

TGFBR2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TGFBR2 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

TGFBR2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGTCGGG GGCTGCTCAG GGGCCTGTGG CCGCTGCACA TCGTCCTGTG GACGCGTATC
61       GCCAGCACGA TCCCACCGCA CGTTCAGAAG TCGGTTAACA ATGACATGAT GGTCACTGAC
121      AACAATGGTG CAGTCAAGTT TCCACAACTG TGTAAATTTT GTGATGTGAG ATTTTCCACC
181      TGTGACAACC AGAAATCCTG CCTGAGCAAC TGCAGCATCA CCTCCATCTG TGAAAAGCCA
241      CAGGAAGTCT GTGTGGCTGT ATGGAGAAAG AATGACGAGA ACATAACACT AGAGACAGTT
301      TGCCATGATC CCAAGCTCCC CTACCATGAC TTTATTCTGG AAGATGCTGC TTCTCCAAAG
361      TGCATTATGA AGGAAAAAAA AAAGCCTGGT GAGACTTTCT TCATGTGTTC CTGTAGCTCT
421      GATGAGTGCA ATGACAACAT CATCTTCTCA GAAGAATACA ACACCAGCAA TCCTGACTTG
481      TTGCTAGTCA TATTTCAAGT GACAGGCATC AGCCTCCTGC CACCACTGGG AGTTGCCATA
541      TCTGTCATCA TCATCTTCTA CTGCTACCGC GTTAACCGGC AGCAGAAGCT GAGCTCATCC
601      TGGGAAGCTG GCAAGCCACG GAAGCTCATG GACTTCAGTG AGCACTGTGC CATCATCCTG
661      GAAGACGACC GCTCCGACAT CAGCTCCACA TGCGCCAACA ACATCAACCA CAACACAGAG
721      CTGCTGCCCA TTGAACTGGA CACCCTGGTG GGGAAAGGTC GCTTTGCTGA GGTCTATAAG
781      GCCAAGCTGA AGCAGAACAC TTCAGAGCAG TTTGAGACAG TGGCAGTCAA GATCTTTCCC
841      TATGAGGAGT ATGCCTCTTG GAAGACGGAG AAGGACATCT TCTCAGACAT CAACCTGAAG
901      CATGAGAACA TACTCCAGTT CCTGACAGCT GAGGAGCGCA AGACGGAGTT GGGGAAACAG
961      TACTGGCTGA TCACTGCCTT CCATGCCAAG GGCAACCTAC AGGAGTACCT GACGCGGCAT
1021     GTCATCAGCT GGGAGGACCT GCGCAAACTG GGCAGCTCCC TCGCCCGGGG CATTGCCCAC
1081     CTCCACAGTG ATCACACTCC GTGCGGGAGG CCCAAGATGC CCATCGTCCA CAGGGACCTC
1141     AAGAGCTCCA ATATCCTCGT GAAGAACGAC CTGACCTGCT GCCTGTGTGA CTTTGGGCTT
1201     TCCCTGCGTC TGGACCCTAC TCTGTCTGTG GATGACCTGG CTAACAGCGG ACAGGTGGGA
1261     ACTGCAAGAT ACATGGCTCC AGAAGTCCTA GAATCCAGGA TGAATTTGGA GAATGTAGAG
1321     TCCTTTAAGC AGACCGATGT CTACTCCATG GCTCTGGTGC TCTGGGAAAT GACATCGCGC
1381     TGTAATGCGG TGGGAGAAGT AAAAGATTAT GAGCCTCCAT TTGGTTCCAA GGTGCGGGAG
1441     CACCCCTGTG TCGAAAGCAT GAAGGACAAC GTGTTGAGAG ATCGAGGACG ACCAGAAATT
1501     CCCAGCTTCT GGCTCAACCA CCAGGGCATC CAGATGGTGT GTGAGACGTT GACTGAGTGC
1561     TGGGACCACG ACCCAGAGGC CCGCCTCACA GCCCAGTGTG TGGCAGAACG CTTCAGTGAG
1621     CTGGAGCACC TGGACAGGCT CTCGGGGAGG AGCTGCTCCG AGGAGAAGAT TCCTGAAGAC
1681     GGCTCCCTAA ACACTACCAA ATAG

 

Quality Control:cDNA sequencing results is available upon request.