Human TGFBR1 Gene cDNA Clone / ORF Clone (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG10459-M	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:  NM_004612.2

cDNA Size:   1512

Synonym:  AAT5; ALK5; SKR4; ALK-5; LDS1A; LDS2A; TGFR-1; ACVRLK4; TGFBR1

Source:  Human.

Description

cDNA Description: ORF Clone of Homo sapiens transforming growth factor, beta receptor 1 DNA.

Vector: Cloning Vector: pMD18-T Simple Vector.

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence.

Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGGAGGCGG CGGTCGCTGC TCCGCGTCCC CGGCTGCTCC TCCTCGTGCT GGCGGCGGCG
61       GCGGCGGCGG CGGCGGCGCT GCTCCCGGGG GCGACGGCGT TACAGTGTTT CTGCCACCTC
121      TGTACAAAAG ACAATTTTAC TTGTGTGACA GATGGGCTCT GCTTTGTCTC TGTCACAGAG
181      ACCACAGACA AAGTTATACA CAACAGCATG TGTATAGCTG AAATTGACTT AATTCCTCGA
241      GATAGGCCGT TTGTATGTGC ACCCTCTTCA AAAACTGGGT CTGTGACTAC AACATATTGC
301      TGCAATCAGG ACCATTGCAA TAAAATAGAA CTTCCAACTA CTGTAAAGTC ATCACCTGGC
361      CTTGGTCCTG TGGAACTGGC AGCTGTCATT GCTGGACCAG TGTGCTTCGT CTGCATCTCA
421      CTCATGTTGA TGGTCTATAT CTGCCACAAC CGCACTGTCA TTCACCATCG AGTGCCAAAT
481      GAAGAGGACC CTTCATTAGA TCGCCCTTTT ATTTCAGAGG GTACTACGTT GAAAGACTTA
541      ATTTATGATA TGACAACGTC AGGTTCTGGC TCAGGTTTAC CATTGCTTGT TCAGAGAACA
601      ATTGCGAGAA CTATTGTGTT ACAAGAAAGC ATTGGCAAAG GTCGATTTGG AGAAGTTTGG
661      AGAGGAAAGT GGCGGGGAGA AGAAGTTGCT GTTAAGATAT TCTCCTCTAG AGAAGAACGT
721      TCGTGGTTCC GTGAGGCAGA GATTTATCAA ACTGTAATGT TACGTCATGA AAACATCCTG
781      GGATTTATAG CAGCAGACAA TAAAGACAAT GGTACTTGGA CTCAGCTCTG GTTGGTGTCA
841      GATTATCATG AGCATGGATC CCTTTTTGAT TACTTAAACA GATACACAGT TACTGTGGAA
901      GGAATGATAA AACTTGCTCT GTCCACGGCG AGCGGTCTTG CCCATCTTCA CATGGAGATT
961      GTTGGTACCC AAGGAAAGCC AGCCATTGCT CATAGAGATT TGAAATCAAA GAATATCTTG
1021     GTAAAGAAGA ATGGAACTTG CTGTATTGCA GACTTAGGAC TGGCAGTAAG ACATGATTCA
1081     GCCACAGATA CCATTGATAT TGCTCCAAAC CACAGAGTGG GAACAAAAAG GTACATGGCC
1141     CCTGAAGTTC TCGATGATTC CATAAATATG AAACATTTTG AATCCTTCAA ACGTGCTGAC
1201     ATCTATGCAA TGGGCTTAGT ATTCTGGGAA ATTGCTCGAC GATGTTCCAT TGGTGGAATT
1261     CATGAAGATT ACCAACTGCC TTATTATGAT CTTGTACCTT CTGACCCATC AGTTGAAGAA
1321     ATGAGAAAAG TTGTTTGTGA ACAGAAGTTA AGGCCAAATA TCCCAAACAG ATGGCAGAGC
1381     TGTGAAGCCT TGAGAGTAAT GGCTAAAATT ATGAGAGAAT GTTGGTATGC CAATGGAGCA
1441     GCTAGGCTTA CAGCATTGCG GATTAAGAAA ACATTATCGC AACTCAGTCA ACAGGAAGGC
1501     ATCAAAATGT AA

Quality Control: cDNA sequencing results is available upon request.

TGFBR1/ALK-5 related areas, pathways, and other information

Cytokine

TGF-beta