TGF-beta 1 Antibody

TGF-beta1 Antibody Datasheet

TGF-beta1 Antibody Product Information

• Immunogen: Recombinant Human TGF-beta1 protein (Catalog#10804-HNAH)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 016
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human TGF-beta 1 (rh TGF-beta 1; Catalog#10804-HNAH; NP_000651.3; Ala 279-Ser 390).

TGF-beta1 Antibody Usage Guide

• Specificity: Human TGF-beta1
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human TGFβ1. The detection limit for Human TGFβ1 is approximately 0.0049 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

TGF-beta1 Antibody Background

Transforming growth factor beta 1 (TGFβ1)is a member of thelarge transforming growth factor beta superfamilyofcytokines, and serves as a multifunctional molecule performing actions in many cellular functions, including the control ofcell growth,cell proliferation,cell differentiation and apoptosis. TGFβ1 was first synthesized as a 390 amino acid precusor. The secreted proprotein contains 360 aa, and is cleaved into mature TGFβ1 (112 aa) and latency-associated peptide (LAP) (248 aa), which remains non-covalently linked to mature TGFβ1 rendering it inactive. TGFβ1 is synthesized by many cells, and regulates many other growth factors both in positive and negative manner. It has been shown that TGFβ1 plays an important role in controlling the immune system and shows different activities on different types of cell, or cells at different developmental stages. It is highly expressed in bone and as a potent stimulator of osteoblastic bone formation. TGFβ1 is able to cause chemotaxis, proliferation and differentiation in committed osteoblasts. TGFβ1 is also identified as a disease marker and a therapeutical target for cardiovascular disease.

References

1. Assoian R, et al., 1983, J Biol Chem.258 (11): 7155-60.
2. Letterio J, et al., 1998,Annu Rev Immunol.16: 137-61.
3. Lebman D, et al., 1999, Microbes Infect.1 (15): 1297-304.
4. Ghadami M, et al., 2000, Am. J. Hum. Genet.66 (1): 143-7.
5. Vaughn SP, et al., 2000, Genomics.66 (1): 119-21.
6. Sanchez-Capelo, A.,2005, Cytokine Growth Factor Rev.16 (1): 15-34.
7. Redondo,S.et al., 2007, Cytokine Growth Factor Rev.18 (3-4): 279-86.