Rat TFRC ORF Expression Clone, His-tagged

TFRC cDNA Clone Datasheet

TFRC cDNA Clone Product Information

• Gene Bank Ref.ID: NM_022712.1
• cDNA Size: 2319
• Gene Synonym: Trfr; Tfrc
• Source: Rat

TFRC cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus transferrin receptor DNA.
• Vector: pCMV/hygro-His
• His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

TFRC cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TFRC cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

TFRC cDNA Clone Sequence and Quality Control

Sequence:

1        ATGATGGATC AAGCCAGATC AGCATTCTCT AACTTGTTTG GAGGGGAGCC ATTGTCATAC
61       ACCCGGTTTA GCCTTGCTCG GCAAGTAGAT GGAGATAACA GTCATGTGGA GATGAAACTG
121      GCTGCAGATG AGGAAGAAAA TGCTGACAGT AACATGAAGG CTAGTGTCAG AAAACCCAAA
181      AGGTTTAATG GAAGACTCTG CTTTGCGACT ATTGCAGTAG TCATCTTTTT TTTGATTGGA
241      TTTATGATCG GCTACCTGGG CTATTGTAAG CGCGTAGAAC AAAAAGAGGA GTGTGTGAGA
301      CTGGCTGAAG CGGAAGAGGC GGACAAGTCA GAAAACGACG AAACAGAATA TGTTCCTAAA
361      TCTTCTCGCT TATTTTGGGC AGACCTCAAA ACACTGTTGT CAGAGAAATT GAATTCCATA
421      GAGTTCACTG ACATCATCAA GCAGCTGAGC CAGAATACAT ATACTCCTCG TGAGGCTGGA
481      TCTCAGAAAG ACGAGAATCT TGCCTATTAT ATTGAAAATC TGTTCCATGA CTTTAAATTC
541      AGCAAAGTCT GGCGAGATGA ACATTATGTG AAGATTCAAG TGAAAAACAG TGTTTCTCAA
601      AACTTGGTGA CCATAAATTC AGGTAGTAAC ATTGACCCAG TGGAGGCTCC TGAGGGTTAT
661      GTGGCATTTA GTAAAGCTGG AGAAGTTACT GGTAAACTGG TCCATGCTAA TTTTGGCACT
721      AAAAAGGACT TTGAAGAATT AAATTATTCT GTGAATGGAT CTTTAGTGAT TGTTAGAGCA
781      GGGAAAATTA CTTTTGCAGA AAAGGTTGCA AATGCCCAAA GCTTTAATGC AATTGGTGTC
841      CTCATCTACA TGGACAGGAA TACGTTCCCC GTTGTTGAGG CAGACCTTCA ATTCTTTGGA
901      CATGCTCATC TAGGAACTGG GGATCCATAT ACACCTGGCT TTCCTTCTTT CAACCATACT
961      CAGTTTCCGC CATCTCAGTC ATCTGGATTG CCTTCTATAC CTGTGCAGAC GATCTCAAGA
1021     GCTGCTGCAG AAAAGCTATT CAAAAACATG GAAGGAAACT GTCCTCCTAG TTGGAATATA
1081     GATTCCTCAT GTAAGCTGGA ACTTTCACAG AATCAAAATG TGAAGCTCAC TGTGAACAAT
1141     GTACTGAAAG AAACAAGAAT ACTTAACATC TTTGGCGTTA TTAAAGGCTA TGAGGAACCA
1201     GACCGCTACA TTGTAGTAGG AGCCCAGAGA GACGCTTGGG GCCCTGGTGT TGCGAAGTCC
1261     AGTGTGGGAA CAGGTCTTCT GTTGAAACTT GCCCAAGTAT TCTCAGATAT GATTTCAAAA
1321     GATGGATTTA GACCCAGCAG GAGTATTATC TTTGCCAGCT GGACTGCAGG AGACTATGGA
1381     GCTGTTGGTG CCACTGAGTG GCTGGAGGGG TACCTTTCAT CTTTGCATCT AAAGGCTTTC
1441     ACTTACATTA ATCTGGATAA AGTCGTCCTG GGTACTAGCA ACTTCAAGGT TTCTGCCAGC
1501     CCCCTATTAT ATACACTTAT GGGGAAGATA ATGCAGGACG TAAAGCATCC GATTGATGGA
1561     AAATATCTAT ATCGAGACAG TAATTGGATT AGCAAAATTG AGGAACTTTC CTTGGACAAT
1621     GCTGCATTCC CTTTTCTTGC ATATTCAGGA ATCCCAGCAG TTTCTTTCTG TTTTTGTGAG
1681     GATGAGGACT ATCCTTATTT GGGCACTAAA CTAGATACCT ATGAGATATT AATTCAGAAA
1741     GTTCCTCAGC TCAACCAAAT GGTTCGTACA GCAGCAGAGG TGGCCGGTCA GTTCATTATT
1801     AAACTTACCC ATGACATTGA GTTGACCCTG GACTATGAGA TGTACAACAG CAAACTACTG
1861     TCATTTATGA AGGATCTGAA CCAGTTCAAA GCAGATATCA AAGATATGGG TCTAAGTCTA
1921     CAATGGCTGT ATTCTGCTCG TGGAGACTAC TTCCGTGCTA CTTCTAGACT AACAACTGAT
1981     TTTCATAATG CTGAGAAAAC AAACAGATTC GTCATGAGGG AAATCAATGA TCGTATTATG
2041     AAAGTGGAGT ATCACTTCCT GTCACCCTAT GTATCTCCAA GAGAGTCTCC TTTCCGACAC
2101     ATCTTCTGGG GCTCTGGCTC TCACACCCTC TCAGCTTTAG TGGAGAACCT GAGACTTCGT
2161     CAGAAAAATA TCACTGCTTT CAATGAAACG CTCTTCAGAA ACCAGTTGGC CCTGGCTACG
2221     TGGACTATTC AGGGAGTTGC AAATGCCCTC TCTGGTGACA TTTGGAATAT TGACAATGAG
2281     TTTGCT1CACC ATCACCACCA TCATCACCAC CATCAC2TAA

 

1. GCT was the nucleotide residue from the restriction site during plasmid construction,
which has no influence on protein expression.
2. His Tag

 

 

Quality Control:cDNA sequencing results is available upon request.