Anti-TFPI2 Antibody

Cat: 10545-RP02
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Anti-TFPI2 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-TFPI2 Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human TFPI2
Immunogen
Recombinant Human TFPI2 protein (Catalog#10545-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human TFPI2 (rh TFPI2; Catalog#10545-H08H; NP_006519.1; Met 1-Lys 213). TFPI2 specific IgG was purified by human TFPI2 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-TFPI2 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:5000-1:10000  
IP 0.2-1 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-TFPI2 Antibody (Rabbit Polyclonal antibody) Images
Human TFPI2 Immunoprecipitation(IP) 8000

TFPI2 was immunoprecipitated using:

Lane A:0.5 mg 293T Whole Cell Lysate

0.5 µL anti-TFPI2 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-TFPI2 rabbit polyclonal antibody,at 1:1000 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 27 kDa

Observed band size: 27 kDa

Human TFPI2 Western blot (WB) 13245

Anti-TFPI2- rabbit polyclonal antibody at 1:500 dilution

Lane A: U87MG Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:27 kDa

Observed band size:27 kDa

(We are unsure as to the identity of these extra bands.)

Anti-TFPI2 Antibody Alternative Names
Anti-PP5 Antibody;Anti-REF1 Antibody;Anti-TFPI-2 Antibody
TFPI2 Background Information

Tissue factor pathway inhibitor-2 (TFPI2), a member of the Kunitz-type serine proteinase inhibitor family, is a structural homologue of tissue factor pathway inhibitor (TFPI). It is a 32 kDa matrix-associated glycoprotein consisting of a short amino-terminal region, three tandem Kunitz-type domains and a positively charged carboxy-terminal tail. TFPI2 inhibits plasmin-dependent activation of several metalloproteinases. TFPI2 is highly abundant in the full-term placenta and widely expressed in various adult human tissues, such as the liver, skeletal muscle, heart, kidney, and pancreas. The expression of TFPI2 in tumors is inversely related to an increasing degree of malignancy, which may suggest a role for TFPI2 in the maintenance of tumor stability and inhibition of the growth of neoplasms. TFPI2 inhibits the tissue factor/factor VIIa (TF/VIIa) complex and a wide variety of serine proteinases including plasmin, plasma kallikrein, factor XIa, trypsin, and chymotrypsin. TFPI2 is involved in regulating pericellular proteases implicated in a variety of physiologic and pathologic processes including cancer cell invasion, vascular inflammation, and atherosclerosis. TFPI2 has also been shown to induce apoptosis and inhibit angiogenesis, which may contribute significantly to tumor growth inhibition.

Full Name
tissue factor pathway inhibitor 2
References
  • Peerschke EI, et al. (2004) Tissue factor pathway inhibitor-2 (TFPI-2) recognizes the complement and kininogen binding protein gC1qR/p33 (gC1qR): implications for vascular inflammation. Thromb Haemost. 92(4): 811-9.
  • Rollin J, et al. (2005) Expression and methylation status of tissue factor pathway inhibitor-2 gene in non-small-cell lung cancer. Br J Cancer. 92(4): 775-83.
  • Chand HS, et al. (2005) Structure, function and biology of tissue factor pathway inhibitor-2. Thromb Haemost. 94(6): 1122-30.
  • Sierko E, et al. (2007) The role of tissue factor pathway inhibitor-2 in cancer biology. Semin Thromb Hemost. 33(7): 653-9.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-TFPI2 Antibody Rabbit Polyclonal ELISA 51002-T16
    Anti-TFPI2 Antibody Rabbit Monoclonal ELISA,ELISA(Cap) 51002-R101
    Anti-TFPI2 Antibody Rabbit Polyclonal ELISA 51002-RP01
    Anti-TFPI2 Antibody Mouse Monoclonal ELISA 10545-MM04
    Anti-TFPI2 Antibody Rabbit Polyclonal ELISA 10545-RP01
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