Human TFF1 ORF Expression Clone, His-tagged

TFF1 cDNA Clone Datasheet

TFF1 cDNA Clone Product Information

• Gene Bank Ref.ID: BC032811
• cDNA Size: 288
• Gene Synonym: pS2;BCEI;HPS2;HP1.A;pNR-2;D21S21;TFF1
• Source: Human

TFF1 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens trefoil factor 1 DNA.
• Vector: pCMV/hygro-His
• His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

TFF1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TFF1 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

TFF1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCACCA TGGAGAACAA GGTGATCTGC GCCCTGGTCC TGGTGTCCAT GCTGGCCCTC
61       GGCACCCTGG CCGAGGCCCA GACAGAGACG TGTACAGTGG CCCCCCGTGA AAGACAGAAT
121      TGTGGTTTTC CTGGTGTCAC GCCCTCCCAG TGTGCAAATA AGGGCTGCTG TTTCGACGAC
181      ACCGTTCGTG GGGTCCCCTG GTGCTTCTAT CCTAATACCA TCGACGTCCC TCCAGAAGAG
241      GAGTGTGAAT TTGCT1CACCA TCACCACCAT CATCACCACC ATCAC2TAA

 

1. GCT was the nucleotide residue from the restriction site during plasmid construction,
which has no influence on protein expression.
2. His Tag

 

 

Quality Control:cDNA sequencing results is available upon request.