TETHERIN / BST2 / CD317 Protein (His Tag)

Bone marrow stromal cell antigen 2 Protein Datasheet

TETHERIN / BST2 / CD317 Protein Product Information

• Synonym: BST2
• Protein Construction: A DNA sequence encoding the human BST2 (Q10589) (Asn49-Ser160) was expressed, with an N-terminal polyhistidine tag.
• Source: Human
• Expression Host: Human Cells

TETHERIN / BST2 / CD317 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: His
• Molecular Mass: The recombinant human BST2 comprises 132 amino acids and has a predicted molecular mass of 14.9 kDa. The apparent molecular mass of the protein is approximately 23-26 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS, pH 7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: TETHERIN / BST2 / CD317 protein

TETHERIN / BST2 / CD317 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

TETHERIN / BST2 / CD317 Protein Description

Tetherin also known as bone marrow stromal antigen 2 and BST2 is single-pass type Ⅱ membrane protein with an extracellular coiled coil domain forms a long semi-flexible rod-like structure important for virion retention at the cell surface and prevention of virus spreading. BST2 predominantly expressed in liver, lung, heart and placenta and low levels in pancreas, kidney, skeletal muscle and brain. This protein is found overexpressed in multiple myeloma cells. It is highly expressed during B-cell development, from pro-B precursors to plasma cells, and on T-cells, monocytes, NK cells and dendritic cells. BST2 is involved in the sorting of secreted proteins and in pre-B cell growth. As an antiretroviral defense protein, BST2 blocks release of enveloped virus from the cell surface. Active against at least four virus families: retroviruses, filoviruses, arenaviruses, and herpesviruses. BST2 is discovered as an inhibitor to HIV-1 infection in the absence of Vpu and it inhibits the release of other viruses such as the Lassa and Marburg virions.

References

1. Ishikawa J. et al., 1995, Genomics. 26 (3): 527-34.
2. Viswanathan K. et al., 2011, PLoS Pathog. 7 (11): 10023-32.
3. Gifford RJ. 2011, Hum Mutat. 32 (11).