Rat TDGF1 Gene cDNA Clone / ORF Clone

TDGF1 cDNA Clone Datasheet

TDGF1 cDNA Clone Product Information

• Gene Bank Ref.ID: XM_001056317.2
• cDNA Size: 546
• Gene Synonym: Tdgf1
• Source: Rat

TDGF1 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus teratocarcinoma-derived growth factor 1 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

TDGF1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TDGF1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

TDGF1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGACTTCT TAACCAGTGT GGTTTTACTT GTGGCCATTT CCAATGCATT TGAATTTGGA
61       CTTGTTGCTG GGATAGGCCG CCGTGAGCTG GCTGTTCCGT CTCCGAGAGA CCTCGACGTC
121      AGAGATAACG ACATTCGGGA CCAGGAAGAA CCTGCACTAC GCCCTCGCTC TCTCCAGCTT
181      GTGCCTTCCG TGGGGATACA GAGCAATAAG TTGCTTAATA AAACTTGCTG TCTGAATGGA
241      GGGACCTGCA TCCTGGGGTC CTTCTGTGCC TGTCCTCCTT CCTTCTACGG ACGCAACTGT
301      GAGCACGATG TACGCAAAGA GCACTGTGGG TCTGTCCTCC ATGGCGCCTG GCTGCCCAAG
361      AAGTGCTCCC TGTGCAGGTG CTGGCACGGC CAGCTCCACT GTTTTCCTCA GACCTTTCTA
421      CCTGGCTGTG ATGGTTACGT GATGGACCAA GACCTCAAGG TATCCAGGAC TCCACGCCAA
481      ACACCGTCTG TGCCAACCAC TGTTATGCTA GCTGGCATCT GCCTTTTCCT AGAAATGAAA
541      GTATAG

 

Quality Control:cDNA sequencing results is available upon request.