TCL1A Protein (His Tag)

T-cell leukemia/lymphoma 1A Protein Datasheet

TCL1A Protein Product Information

• Synonym: TCL1
• Protein Construction: A DNA sequence encoding the human TCL1A (P56279) (Ala 2-Asp 114) was expressed, with a polyhistide tag at the N-terminus.
• Source: Human
• Expression Host: E. Coli

TCL1A Protein QC Testing

• Purity: > 88 % as determined by SDS-PAGE.
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human TCL1A consisting of 128 amino acids and has a calculated molecular mass of 15.2 KDa. It migrates as an 22 kDa band in SDS-PAGE under reducing conditions as predicted.
• Formulation: Lyophilized from sterile PBS, pH7.5
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: TCL1A protein

TCL1A Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

TCL1A Protein Description

T-cell leukemia/lymphoma 1 (TCL1) protooncogene is expressed in CD3^- CD4^- CD8^- precursor T cells and is extinguished at the CD4⁺ CD8⁺ stage of thymocyte development. In B cells, TCL1 is first expressed in pro-B cells and remains high in naive mantle zone B cells of peripheral lymphoid tissues. The TCL1 protooncogene is overexpressed in many mature B cell lymphomas, especially from AIDS patients. It has been demonstrated that TCL1 is a powerful oncogene that, when overexpressed in both B and T cells, predominantly yields mature B cell lymphomas. All TCL1 isoforms bind to the Akt pleckstrin homology domain. Both in vitro and in vivo TCL1 increases Akt kinase activity and as a consequence enhances substrate phosphorylation. In vivo, TCL1 stabilizes the mitochondrial transmembrane potential and enhances cell proliferation and survival. It has been shown that TCL1 is a novel Akt kinase coactivator, which promotes Akt-induced cell survival and proliferation. T-cell leukemia / lymphoma protein 1A (TCL1A) is a member of the TCL1 family.

References

1. Jarmo Laine. et al., 2000, Molecular Cell. 6 (2): 395-407.
2. Virgilio L. et al., 1994, PNAS December. 91 (26): 12530-4.
3. Hoyer KK. et al., 2002, Proc Natl Acad Sci. 99 (22): 14392-7.