Human TBP transcript variant 1 ORF Expression Clone, untagged

TBP cDNA Clone Datasheet

TBP cDNA Clone Product Information

• Gene Bank Ref.ID: NM_003194.4
• cDNA Size: 1017
• Gene Synonym: GTF2D; SCA17; TFIID; GTF2D1; MGC117320; MGC126054; MGC126055; TBP
• Source: Human

TBP cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens TATA box binding protein, transcript variant DNA.
• Vector: pCMV/hygro
• Sequence Description: Same as Gene Bank Ref. ID sequence with the nucleotide (216-218) deletion mutation and one point mutation 228 G/A not causing the amino acid variation.

TBP cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TBP cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

TBP cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGATCAGA ACAACAGCCT GCCACCTTAC GCTCAGGGCT TGGCCTCCCC TCAGGGTGCC
61       ATGACTCCCG GAATCCCTAT CTTTAGTCCA ATGATGCCTT ATGGCACTGG ACTGACCCCA
121      CAGCCTATTC AGAACACCAA TAGTCTGTCT ATTTTGGAAG AGCAACAAAG GCAGCAGCAG
181      CAACAACAAC AACAGCAGCA GCAGCAGCAG CAGCAGCAAC AGCAGCAGCA GCAGCAGCAG
241      CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAAC AGGCAGTGGC AGCTGCAGCC
301      GTTCAGCAGT CAACGTCCCA GCAGGCAACA CAGGGAACCT CAGGCCAGGC ACCACAGCTC
361      TTCCACTCAC AGACTCTCAC AACTGCACCC TTGCCGGGCA CCACTCCACT GTATCCCTCC
421      CCCATGACTC CCATGACCCC CATCACTCCT GCCACGCCAG CTTCGGAGAG TTCTGGGATT
481      GTACCGCAGC TGCAAAATAT TGTATCCACA GTGAATCTTG GTTGTAAACT TGACCTAAAG
541      ACCATTGCAC TTCGTGCCCG AAACGCCGAA TATAATCCCA AGCGGTTTGC TGCGGTAATC
601      ATGAGGATAA GAGAGCCACG AACCACGGCA CTGATTTTCA GTTCTGGGAA AATGGTGTGC
661      ACAGGAGCCA AGAGTGAAGA ACAGTCCAGA CTGGCAGCAA GAAAATATGC TAGAGTTGTA
721      CAGAAGTTGG GTTTTCCAGC TAAGTTCTTG GACTTCAAGA TTCAGAATAT GGTGGGGAGC
781      TGTGATGTGA AGTTTCCTAT AAGGTTAGAA GGCCTTGTGC TCACCCACCA ACAATTTAGT
841      AGTTATGAGC CAGAGTTATT TCCTGGTTTA ATCTACAGAA TGATCAAACC CAGAATTGTT
901      CTCCTTATTT TTGTTTCTGG AAAAGTTGTA TTAACAGGTG CTAAAGTCAG AGCAGAAATT
961      TATGAAGCAT TTGAAAACAT CTACCCTATT CTAAAGGGAT TCAGGAAGAC GACGTAA

 

 

Quality Control:cDNA sequencing results is available upon request.